Enhancement of cell adhesion and spreading by a cartilage-specific noncollagenous protein, cartilage matrix protein (CMP/Matrilin-1), via integrin alpha1beta1 - PubMed (original) (raw)
. 1999 Apr 16;274(16):11417-23.
doi: 10.1074/jbc.274.16.11417.
Affiliations
- PMID: 10196235
- DOI: 10.1074/jbc.274.16.11417
Free article
Enhancement of cell adhesion and spreading by a cartilage-specific noncollagenous protein, cartilage matrix protein (CMP/Matrilin-1), via integrin alpha1beta1
S Makihira et al. J Biol Chem. 1999.
Free article
Abstract
Cartilage matrix protein (CMP; also known as matrilin-1), one of the major noncollagenous proteins in most cartilages, binds to aggrecan and type II collagen. We examined the effect of CMP on the adhesion of chondrocytes and fibroblasts using CMP-coated dishes. The CMP coating at 10-20 micrograms/ml enhanced the adhesion and spreading of rabbit growth plate, resting and articular chondrocytes, and fibroblasts and human epiphyseal chondrocytes and MRC5 fibroblasts. The effect of CMP on the spreading of chondrocytes was synergistically increased by native, but not heated, type II collagen (gelatin). The monoclonal antibody to integrin alpha1 or beta1 abolished CMP-induced cell adhesion and spreading, whereas the antibody to integrin alpha2, alpha3, alpha5, beta2, alpha5beta1, or alphaVbeta5 had little effect on cell adhesion or spreading. The antibody to integrin alpha1, but not to other subunits, coprecipitated 125I-CMP that was added to MRC5 cell lysates, indicating the association of CMP with the integrin alpha1 subunit. Unlabeled CMP competed for the binding to integrin alpha1 with 125I-CMP. These findings suggest that CMP is a potent adhesion factor for chondrocytes, particularly in the presence of type II collagen, and that integrin alpha1beta1 is involved in CMP-mediated cell adhesion and spreading. Since CMP is expressed almost exclusively in cartilage, this adhesion factor, unlike fibronectin or laminin, may play a special role in the development and remodeling of cartilage.
Similar articles
- Integrins and chondrocyte-matrix interactions in articular cartilage.
Loeser RF. Loeser RF. Matrix Biol. 2014 Oct;39:11-6. doi: 10.1016/j.matbio.2014.08.007. Epub 2014 Aug 25. Matrix Biol. 2014. PMID: 25169886 Free PMC article. Review. - RGD-CAP ((beta)ig-h3) enhances the spreading of chondrocytes and fibroblasts via integrin alpha(1)beta(1).
Ohno S, Noshiro M, Makihira S, Kawamoto T, Shen M, Yan W, Kawashima-Ohya Y, Fujimoto K, Tanne K, Kato Y. Ohno S, et al. Biochim Biophys Acta. 1999 Aug 12;1451(1):196-205. doi: 10.1016/s0167-4889(99)00093-2. Biochim Biophys Acta. 1999. PMID: 10446401 - Cartilage oligomeric matrix protein/thrombospondin 5 supports chondrocyte attachment through interaction with integrins.
Chen FH, Thomas AO, Hecht JT, Goldring MB, Lawler J. Chen FH, et al. J Biol Chem. 2005 Sep 23;280(38):32655-61. doi: 10.1074/jbc.M504778200. Epub 2005 Jul 28. J Biol Chem. 2005. PMID: 16051604 Free PMC article.
Cited by
- Digits in a dish: An in vitro system to assess the molecular genetics of hand/foot development at single-cell resolution.
Fuiten AM, Yoshimoto Y, Shukunami C, Stadler HS. Fuiten AM, et al. Front Cell Dev Biol. 2023 Mar 13;11:1135025. doi: 10.3389/fcell.2023.1135025. eCollection 2023. Front Cell Dev Biol. 2023. PMID: 36994104 Free PMC article. - Translational applications of photopolymerizable hydrogels for cartilage repair.
Meng W, Gao L, Venkatesan JK, Wang G, Madry H, Cucchiarini M. Meng W, et al. J Exp Orthop. 2019 Dec 5;6(1):47. doi: 10.1186/s40634-019-0215-3. J Exp Orthop. 2019. PMID: 31807962 Free PMC article. Review. - Von Willebrand Factor Type A domain of hCLCA1 is sufficient for U-937 macrophage activation.
Keith BA, Ching JCH, Loewen ME. Keith BA, et al. Biochem Biophys Rep. 2019 Mar 28;18:100630. doi: 10.1016/j.bbrep.2019.100630. eCollection 2019 Jul. Biochem Biophys Rep. 2019. PMID: 30984882 Free PMC article. - Integrins and chondrocyte-matrix interactions in articular cartilage.
Loeser RF. Loeser RF. Matrix Biol. 2014 Oct;39:11-6. doi: 10.1016/j.matbio.2014.08.007. Epub 2014 Aug 25. Matrix Biol. 2014. PMID: 25169886 Free PMC article. Review. - Matrilin-2 is proteolytically cleaved by ADAMTS-4 and ADAMTS-5.
Wang Z, Luo J, Iwamoto S, Chen Q. Wang Z, et al. Molecules. 2014 Jun 23;19(6):8472-87. doi: 10.3390/molecules19068472. Molecules. 2014. PMID: 24959676 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials