Essential role of LAT in T cell development - PubMed (original) (raw)

doi: 10.1016/s1074-7613(00)80032-1.

C L Sommers, D N Burshtyn, C C Stebbins, J B DeJarnette, R P Trible, A Grinberg, H C Tsay, H M Jacobs, C M Kessler, E O Long, P E Love, L E Samelson

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• PMID: 10204488
• DOI: 10.1016/s1074-7613(00)80032-1

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Essential role of LAT in T cell development

W Zhang et al. Immunity. 1999 Mar.

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Abstract

The linker molecule LAT is a substrate of the tyrosine kinases activated following TCR engagement. Phosphorylated LAT binds many critical signaling molecules. The central role of this molecule in TCR-mediated signaling has been demonstrated by experiments in a LAT-deficient cell line. To probe the role of LAT in T cell development, the LAT gene was disrupted by targeting. LAT-deficient mice appeared healthy. Flow cytometric analysis revealed normal B cell populations but the absence of any mature peripheral T cells. Intrathymic development was blocked within the CD4- CD8- stage. No gross abnormality of NK or platelet function was observed. LAT is thus critical to both T cell activation and development.

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