Conformation of the Bax C-terminus regulates subcellular location and cell death - PubMed (original) (raw)
Conformation of the Bax C-terminus regulates subcellular location and cell death
A Nechushtan et al. EMBO J. 1999.
Abstract
Bax, a pro-apoptotic member of the Bcl-2 family, translocates from the cytosol to the mitochondria during programmed cell death. We report here that both gain-of-function and loss-of-function mutations can be achieved by altering a single amino acid in the Bax hydrophobic C-terminus. The properly mutated C-terminus of Bax can target a non-relevant protein to the mitochondria, showing that specific conformations of this domain alone allow mitochondrial docking. These data along with N-terminus epitope exposure experiments suggest that the C- and the N-termini interact and that upon triggering of apoptosis, Bax changes conformation, exposing these two domains to insert into the mitochondria and regulate the cell death machinery.
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References
- Br J Cancer. 1972 Aug;26(4):239-57 - PubMed
- Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3668-72 - PubMed
- Cancer Res. 1993 Oct 1;53(19):4701-14 - PubMed
- Cell. 1993 Oct 22;75(2):241-51 - PubMed
- J Biol Chem. 1993 Dec 5;268(34):25265-8 - PubMed
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