Action of DNA repair endonuclease ERCC1/XPF in living cells - PubMed (original) (raw)

Action of DNA repair endonuclease ERCC1/XPF in living cells

A B Houtsmuller et al. Science. 1999.

Abstract

To study the nuclear organization and dynamics of nucleotide excision repair (NER), the endonuclease ERCC1/XPF (for excision repair cross complementation group 1/xeroderma pigmentosum group F) was tagged with green fluorescent protein and its mobility was monitored in living Chinese hamster ovary cells. In the absence of DNA damage, the complex moved freely through the nucleus, with a diffusion coefficient (15 +/- 5 square micrometers per second) consistent with its molecular size. Ultraviolet light-induced DNA damage caused a transient dose-dependent immobilization of ERCC1/XPF, likely due to engagement of the complex in a single repair event. After 4 minutes, the complex regained mobility. These results suggest (i) that NER operates by assembly of individual NER factors at sites of DNA damage rather than by preassembly of holocomplexes and (ii) that ERCC1/XPF participates in repair of DNA damage in a distributive fashion rather than by processive scanning of large genome segments.

PubMed Disclaimer

Similar articles

• Mislocalization of XPF-ERCC1 nuclease contributes to reduced DNA repair in XP-F patients.
  Ahmad A, Enzlin JH, Bhagwat NR, Wijgers N, Raams A, Appledoorn E, Theil AF, J Hoeijmakers JH, Vermeulen W, J Jaspers NG, Schärer OD, Niedernhofer LJ. Ahmad A, et al. PLoS Genet. 2010 Mar 5;6(3):e1000871. doi: 10.1371/journal.pgen.1000871. PLoS Genet. 2010. PMID: 20221251 Free PMC article.
• The formation of UV-induced chromosome aberrations involves ERCC1 and XPF but not other nucleotide excision repair genes.
  Chipchase MD, Melton DW. Chipchase MD, et al. DNA Repair (Amst). 2002 Apr 29;1(4):335-40. doi: 10.1016/s1568-7864(02)00010-1. DNA Repair (Amst). 2002. PMID: 12509251
• Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH.
  Zotter A, Luijsterburg MS, Warmerdam DO, Ibrahim S, Nigg A, van Cappellen WA, Hoeijmakers JH, van Driel R, Vermeulen W, Houtsmuller AB. Zotter A, et al. Mol Cell Biol. 2006 Dec;26(23):8868-79. doi: 10.1128/MCB.00695-06. Epub 2006 Sep 25. Mol Cell Biol. 2006. PMID: 17000769 Free PMC article.
• Function and Interactions of ERCC1-XPF in DNA Damage Response.
  Faridounnia M, Folkers GE, Boelens R. Faridounnia M, et al. Molecules. 2018 Dec 5;23(12):3205. doi: 10.3390/molecules23123205. Molecules. 2018. PMID: 30563071 Free PMC article. Review.
• Interstrand crosslink repair: can XPF-ERCC1 be let off the hook?
  Bergstralh DT, Sekelsky J. Bergstralh DT, et al. Trends Genet. 2008 Feb;24(2):70-6. doi: 10.1016/j.tig.2007.11.003. Epub 2008 Jan 14. Trends Genet. 2008. PMID: 18192062 Review.

Cited by

• Small Molecule Antagonists of the DNA Repair ERCC1/XPA Protein-Protein Interaction.
  Obermann R, Yemane B, Jarvis C, Franco FM, Kyriukha Y, Nolan W, Gohara B, Krezel AM, Wildman SA, Janetka JW. Obermann R, et al. ChemMedChem. 2024 Apr 16;19(8):e202300648. doi: 10.1002/cmdc.202300648. Epub 2024 Mar 5. ChemMedChem. 2024. PMID: 38300970
• Compartmentalization of androgen receptors at endogenous genes in living cells.
  Yavuz S, Kabbech H, van Staalduinen J, Linder S, van Cappellen WA, Nigg AL, Abraham TE, Slotman JA, Quevedo M, Poot RA, Zwart W, van Royen ME, Grosveld FG, Smal I, Houtsmuller AB. Yavuz S, et al. Nucleic Acids Res. 2023 Nov 10;51(20):10992-11009. doi: 10.1093/nar/gkad803. Nucleic Acids Res. 2023. PMID: 37791849 Free PMC article.
• Revisiting the Anti-Cancer Toxicity of Clinically Approved Platinating Derivatives.
  Forgie BN, Prakash R, Telleria CM. Forgie BN, et al. Int J Mol Sci. 2022 Dec 6;23(23):15410. doi: 10.3390/ijms232315410. Int J Mol Sci. 2022. PMID: 36499737 Free PMC article. Review.
• The Role of Single-Nucleotide Polymorphisms in Cholangiocarcinoma: A Systematic Review.
  Wang G, Heij LR, Liu D, Dahl E, Lang SA, Ulmer TF, Luedde T, Neumann UP, Bednarsch J. Wang G, et al. Cancers (Basel). 2022 Dec 2;14(23):5969. doi: 10.3390/cancers14235969. Cancers (Basel). 2022. PMID: 36497451 Free PMC article. Review.
• Vascular Ageing Features Caused by Selective DNA Damage in Smooth Muscle Cell.
  Ataei Ataabadi E, Golshiri K, van der Linden J, de Boer M, Duncker DJ, Jüttner A, de Vries R, Van Veghel R, van der Pluijm I, Dutheil S, Chalgeri S, Zhang L, Lin A, Davis RE, Snyder GL, Danser AHJ, Roks AJM. Ataei Ataabadi E, et al. Oxid Med Cell Longev. 2021 Aug 31;2021:2308317. doi: 10.1155/2021/2308317. eCollection 2021. Oxid Med Cell Longev. 2021. PMID: 34504640 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Molecular Biology Databases

  • BioCyc