HOX11 interacts with CTF1 and mediates hematopoietic precursor cell immortalization - PubMed (original) (raw)

Comparative Study

. 1999 Apr 1;18(13):2273-9.

doi: 10.1038/sj.onc.1202545.

Affiliations

Comparative Study

HOX11 interacts with CTF1 and mediates hematopoietic precursor cell immortalization

N Zhang et al. Oncogene. 1999.

Abstract

HOX11 is a homeodomain-containing oncogenic transcription factor that immortalizes hematopoietic precursor cells. The mechanism by which HOX11 facilitates this initial step of leukemogenesis is, however, not well understood. We have used a DNA binding site selection assay to investigate cooperative DNA binding by HOX11 with other transcription factors. A consensus sequence was derived and identified as the binding site for the CCAAT-box-binding transcription factors (CTF). HOX11 was shown to interact in vitro and in vivo with CTF1. Retrovirus-mediated transduction of an antisense CTF1 cDNA dramatically reduced the proliferative capacity of HOX11-immortalized hematopoietic precursor cells. CTF1 is, therefore, the first HOX11 protein partner identified that plays an important role in hematopoietic precursor cell immortalization.

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