PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice - PubMed (original) (raw)

PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice

A J Raeber et al. EMBO J. 1999.

Abstract

An intact immune system, and particularly the presence of mature B lymphocytes, is crucial for mouse scrapie pathogenesis in the brain after peripheral exposure. Prions are accumulated in the lymphoreticular system (LRS), but the identity of the cells containing infectivity and their role in neuroinvasion have not been determined. We show here that although prion infectivity in the spleen is associated with B and T lymphocytes and to a lesser degree with the stroma, no infectivity could be detected in lymphocytes from blood. In wild-type mice, which had been irradiated and reconstituted with PrP-deficient lymphohaematopoietic stem cells and inoculated with scrapie prions, infectivity in the spleen was present in the stroma but not in lymphocytes. Therefore, splenic B and T lymphocytes can either synthesize prions or acquire them from another source, but only when they express PrP.

PubMed Disclaimer

Similar articles

• Prions and the lymphoreticular system.
  Weissmann C, Raeber AJ, Montrasio F, Hegyi I, Frigg R, Klein MA, Aguzzi A. Weissmann C, et al. Philos Trans R Soc Lond B Biol Sci. 2001 Feb 28;356(1406):177-84. doi: 10.1098/rstb.2000.0763. Philos Trans R Soc Lond B Biol Sci. 2001. PMID: 11260798 Free PMC article. Review.
• Studies on prion replication in spleen.
  Raeber AJ, Montrasio F, Hegyi I, Frigg R, Klein MA, Aguzzi A, Weissmann C. Raeber AJ, et al. Dev Immunol. 2001;8(3-4):291-304. doi: 10.1155/2001/95404. Dev Immunol. 2001. PMID: 11785678 Free PMC article.
• Efficient lymphoreticular prion propagation requires PrP(c) in stromal and hematopoietic cells.
  Kaeser PS, Klein MA, Schwarz P, Aguzzi A. Kaeser PS, et al. J Virol. 2001 Aug;75(15):7097-106. doi: 10.1128/JVI.75.15.7097-7106.2001. J Virol. 2001. PMID: 11435590 Free PMC article.
• PrP-expressing tissue required for transfer of scrapie infectivity from spleen to brain.
  Blättler T, Brandner S, Raeber AJ, Klein MA, Voigtländer T, Weissmann C, Aguzzi A. Blättler T, et al. Nature. 1997 Sep 4;389(6646):69-73. doi: 10.1038/37981. Nature. 1997. PMID: 9288968
• Susceptibility to scrapie in mice is dependent on PrPC.
  Weissmann C, Büeler H, Fischer M, Sauer A, Aguet M. Weissmann C, et al. Philos Trans R Soc Lond B Biol Sci. 1994 Mar 29;343(1306):431-3. doi: 10.1098/rstb.1994.0040. Philos Trans R Soc Lond B Biol Sci. 1994. PMID: 7913762 Review.

Cited by

• Infected splenic dendritic cells are sufficient for prion transmission to the CNS in mouse scrapie.
  Aucouturier P, Geissmann F, Damotte D, Saborio GP, Meeker HC, Kascsak R, Kascsak R, Carp RI, Wisniewski T. Aucouturier P, et al. J Clin Invest. 2001 Sep;108(5):703-8. doi: 10.1172/JCI13155. J Clin Invest. 2001. PMID: 11544275 Free PMC article.
• Transmission of prions.
  Weissmann C, Enari M, Klöhn PC, Rossi D, Flechsig E. Weissmann C, et al. Proc Natl Acad Sci U S A. 2002 Dec 10;99 Suppl 4(Suppl 4):16378-83. doi: 10.1073/pnas.172403799. Epub 2002 Aug 14. Proc Natl Acad Sci U S A. 2002. PMID: 12181490 Free PMC article.
• Microglia from Creutzfeldt-Jakob disease-infected brains are infectious and show specific mRNA activation profiles.
  Baker CA, Martin D, Manuelidis L. Baker CA, et al. J Virol. 2002 Nov;76(21):10905-13. doi: 10.1128/jvi.76.21.10905-10913.2002. J Virol. 2002. PMID: 12368333 Free PMC article.
• Epithelial and endothelial expression of the green fluorescent protein reporter gene under the control of bovine prion protein (PrP) gene regulatory sequences in transgenic mice.
  Lemaire-Vieille C, Schulze T, Podevin-Dimster V, Follet J, Bailly Y, Blanquet-Grossard F, Decavel JP, Heinen E, Cesbron JY. Lemaire-Vieille C, et al. Proc Natl Acad Sci U S A. 2000 May 9;97(10):5422-7. doi: 10.1073/pnas.080081197. Proc Natl Acad Sci U S A. 2000. PMID: 10792029 Free PMC article.
• Post-natal knockout of prion protein alters hippocampal CA1 properties, but does not result in neurodegeneration.
  Mallucci GR, Ratté S, Asante EA, Linehan J, Gowland I, Jefferys JG, Collinge J. Mallucci GR, et al. EMBO J. 2002 Feb 1;21(3):202-10. doi: 10.1093/emboj/21.3.202. EMBO J. 2002. PMID: 11823413 Free PMC article.

References

1. 1. J Infect Dis. 1967 Feb;117(1):15-22 - PubMed
2. 1. J Pathol. 1992 Dec;168(4):413-8 - PubMed
3. 1. Nature. 1970 May 2;226(5244):462-3 - PubMed
4. 1. Nature. 1970 Nov 21;228(5273):779-80 - PubMed
5. 1. J Gen Virol. 1980 Nov;51(Pt 1):183-7 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central

• Research Materials

  • NCI CPTC Antibody Characterization Program