Mammalian telomeres end in a large duplex loop - PubMed (original) (raw)

Mammalian telomeres end in a large duplex loop

J D Griffith et al. Cell. 1999.

Free article

Abstract

Mammalian telomeres contain a duplex array of telomeric repeats bound to the telomeric repeat-binding factors TRF1 and TRF2. Inhibition of TRF2 results in immediate deprotection of chromosome ends, manifested by loss of the telomeric 3' overhang, activation of p53, and end-to-end chromosome fusions. Electron microscopy reported here demonstrated that TRF2 can remodel linear telomeric DNA into large duplex loops (t loops) in vitro. Electron microscopy analysis of psoralen cross-linked telomeric DNA purified from human and mouse cells revealed abundant large t loops with a size distribution consistent with their telomeric origin. Binding of TRF1 and single strand binding protein suggested that t loops are formed by invasion of the 3' telomeric overhang into the duplex telomeric repeat array. T loops may provide a general mechanism for the protection and replication of telomeres.

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Comment in

• Telomeres do D-loop-T-loop.
  Greider CW. Greider CW. Cell. 1999 May 14;97(4):419-22. doi: 10.1016/s0092-8674(00)80750-3. Cell. 1999. PMID: 10338204 Review. No abstract available.

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