Two-dimensional map of human brain proteins - PubMed (original) (raw)
Two-dimensional map of human brain proteins
H Langen et al. Electrophoresis. 1999 Apr-May.
Abstract
Samples of human brain from the parietal cortex lobe were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradient strips covering the various pH regions. The protein spots were visualized with colloidal Coomassie blue stain and identified by matrix-assisted laser desorption/ionization mass spectrometry. Approximately 400 spots were identified, corresponding to 180 different brain proteins. The list of identified proteins includes a large number of structural proteins and of enzymes or enzyme subunits with various catalytic activities. The majority of proteins are localized in the cytoplasma and in mitochondria. The two-dimensional map may be useful as a reference database to study changes in the protein level caused by various disorders, such as Alzheimer's disease, major depression and schizophrenia.
Similar articles
- Rat brain proteins: two-dimensional protein database and variations in the expression level.
Fountoulakis M, Schuller E, Hardmeier R, Berndt P, Lubec G. Fountoulakis M, et al. Electrophoresis. 1999 Dec;20(18):3572-9. doi: 10.1002/(SICI)1522-2683(19991201)20:18<3572::AID-ELPS3572>3.0.CO;2-T. Electrophoresis. 1999. PMID: 10612283 - Two-dimensional map of basic proteins of Haemophilus influenzae.
Fountoulakis M, Takács B, Langen H. Fountoulakis M, et al. Electrophoresis. 1998 May;19(5):761-6. doi: 10.1002/elps.1150190527. Electrophoresis. 1998. PMID: 9629912 - Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a two-dimensional polyacrylamide gel electrophoresis database.
Shaw AC, Røssel Larsen M, Roepstorff P, Holm A, Christiansen G, Birkelund S. Shaw AC, et al. Electrophoresis. 1999 Apr-May;20(4-5):977-83. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<977::AID-ELPS977>3.0.CO;2-J. Electrophoresis. 1999. PMID: 10344275 - Postmortem changes in the level of brain proteins.
Fountoulakis M, Hardmeier R, Höger H, Lubec G. Fountoulakis M, et al. Exp Neurol. 2001 Jan;167(1):86-94. doi: 10.1006/exnr.2000.7529. Exp Neurol. 2001. PMID: 11161596 - Perspectives on immobilized proteins.
Birnbaum S, Mosbach K. Birnbaum S, et al. Curr Opin Biotechnol. 1991 Feb;2(1):44-51. doi: 10.1016/0958-1669(91)90060-i. Curr Opin Biotechnol. 1991. PMID: 1367709 Review. No abstract available.
Cited by
- Misfolded Protein Linked Strategies Toward Biomarker Development for Neurodegenerative Diseases.
Kumar S, Karthikeyan N, Mishra S, Padmanabhan P, Radda G, Gulyás B. Kumar S, et al. Mol Neurobiol. 2019 Apr;56(4):2559-2578. doi: 10.1007/s12035-018-1232-4. Epub 2018 Jul 24. Mol Neurobiol. 2019. PMID: 30043261 Review. - Approaches for targeted proteomics and its potential applications in neuroscience.
Sethi S, Chourasia D, Parhar IS. Sethi S, et al. J Biosci. 2015 Sep;40(3):607-27. doi: 10.1007/s12038-015-9537-1. J Biosci. 2015. PMID: 26333406 Review. - Identifying changes in the synaptic proteome of cirrhotic alcoholic superior frontal gyrus.
Etheridge N, Mayfield RD, Harris RA, Dodd PR. Etheridge N, et al. Curr Neuropharmacol. 2011 Mar;9(1):122-8. doi: 10.2174/157015911795017164. Curr Neuropharmacol. 2011. PMID: 21886576 Free PMC article. - Synaptic proteome changes in the superior frontal gyrus and occipital cortex of the alcoholic brain.
Etheridge N, Lewohl JM, Mayfield RD, Harris RA, Dodd PR. Etheridge N, et al. Proteomics Clin Appl. 2009 Jun 24;3(6):730-742. doi: 10.1002/prca.200800202. Proteomics Clin Appl. 2009. PMID: 19924264 Free PMC article. - Oral administration of circulating precursors for membrane phosphatides can promote the synthesis of new brain synapses.
Cansev M, Wurtman RJ, Sakamoto T, Ulus IH. Cansev M, et al. Alzheimers Dement. 2008 Jan;4(1 Suppl 1):S153-68. doi: 10.1016/j.jalz.2007.10.005. Epub 2007 Dec 21. Alzheimers Dement. 2008. PMID: 18631994 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources