Putative reaction intermediates in Crm1-mediated nuclear protein export - PubMed (original) (raw)
. 1999 Jun 4;274(23):16279-86.
doi: 10.1074/jbc.274.23.16279.
Affiliations
- PMID: 10347184
- DOI: 10.1074/jbc.274.23.16279
Free article
Putative reaction intermediates in Crm1-mediated nuclear protein export
M Floer et al. J Biol Chem. 1999.
Free article
Abstract
We discovered several novel interactions between proteins involved in Crm1-mediated nuclear export of the nuclear export signal containing human immunodeficiency virus type 1 protein Rev. First, a Rev/Crm1/RanGTP complex (where Ran is Ras-related nuclear protein) reacts with some nucleoporins (Nup42 and Nup159) but not others (NSP1, Nup116, and Nup1), forming a Nup/Crm1/RanGTP complex and concomitantly releasing Rev. Second, RanBP1 (or homologous proteins) can displace Nup and form a ternary RanBP1/RanGTP/Crm1 complex that can be disassembled by RanGAP via GTP hydrolysis. Third, and most surprisingly, RanBP1/RanGTP/Crm1 can be disassembled without GTP hydrolysis by the nucleotide exchange factor RanGEF. Recycling of a Ran/RanGEF complex by GTP and Mg2+ is stimulated by both Crm1 and Rev, allowing reformation of a Rev/Crm1/RanGTP complex. Based on these reactions we propose a model for Crm1-mediated export.
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