Putative reaction intermediates in Crm1-mediated nuclear protein export - PubMed (original) (raw)
. 1999 Jun 4;274(23):16279-86.
doi: 10.1074/jbc.274.23.16279.
Affiliations
- PMID: 10347184
- DOI: 10.1074/jbc.274.23.16279
Free article
Putative reaction intermediates in Crm1-mediated nuclear protein export
M Floer et al. J Biol Chem. 1999.
Free article
Abstract
We discovered several novel interactions between proteins involved in Crm1-mediated nuclear export of the nuclear export signal containing human immunodeficiency virus type 1 protein Rev. First, a Rev/Crm1/RanGTP complex (where Ran is Ras-related nuclear protein) reacts with some nucleoporins (Nup42 and Nup159) but not others (NSP1, Nup116, and Nup1), forming a Nup/Crm1/RanGTP complex and concomitantly releasing Rev. Second, RanBP1 (or homologous proteins) can displace Nup and form a ternary RanBP1/RanGTP/Crm1 complex that can be disassembled by RanGAP via GTP hydrolysis. Third, and most surprisingly, RanBP1/RanGTP/Crm1 can be disassembled without GTP hydrolysis by the nucleotide exchange factor RanGEF. Recycling of a Ran/RanGEF complex by GTP and Mg2+ is stimulated by both Crm1 and Rev, allowing reformation of a Rev/Crm1/RanGTP complex. Based on these reactions we propose a model for Crm1-mediated export.
Similar articles
- The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP.
Askjaer P, Jensen TH, Nilsson J, Englmeier L, Kjems J. Askjaer P, et al. J Biol Chem. 1998 Dec 11;273(50):33414-22. doi: 10.1074/jbc.273.50.33414. J Biol Chem. 1998. PMID: 9837918 - CRM1 is an export receptor for leucine-rich nuclear export signals.
Fornerod M, Ohno M, Yoshida M, Mattaj IW. Fornerod M, et al. Cell. 1997 Sep 19;90(6):1051-60. doi: 10.1016/s0092-8674(00)80371-2. Cell. 1997. PMID: 9323133 - Atomic basis of CRM1-cargo recognition, release and inhibition.
Fung HY, Chook YM. Fung HY, et al. Semin Cancer Biol. 2014 Aug;27:52-61. doi: 10.1016/j.semcancer.2014.03.002. Epub 2014 Mar 12. Semin Cancer Biol. 2014. PMID: 24631835 Free PMC article. Review. - The HIV-1 Rev protein.
Pollard VW, Malim MH. Pollard VW, et al. Annu Rev Microbiol. 1998;52:491-532. doi: 10.1146/annurev.micro.52.1.491. Annu Rev Microbiol. 1998. PMID: 9891806 Review.
Cited by
- Nuclear transport proteins: structure, function, and disease relevance.
Yang Y, Guo L, Chen L, Gong B, Jia D, Sun Q. Yang Y, et al. Signal Transduct Target Ther. 2023 Nov 10;8(1):425. doi: 10.1038/s41392-023-01649-4. Signal Transduct Target Ther. 2023. PMID: 37945593 Free PMC article. Review. - RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases.
Audia S, Brescia C, Dattilo V, D'Antona L, Calvano P, Iuliano R, Trapasso F, Perrotti N, Amato R. Audia S, et al. Cancers (Basel). 2023 Jan 12;15(2):486. doi: 10.3390/cancers15020486. Cancers (Basel). 2023. PMID: 36672435 Free PMC article. Review. - Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals.
Li Y, Zhou J, Min S, Zhang Y, Zhang Y, Zhou Q, Shen X, Jia D, Han J, Sun Q. Li Y, et al. Elife. 2019 Apr 25;8:e41331. doi: 10.7554/eLife.41331. Elife. 2019. PMID: 31021318 Free PMC article. - RNA biology of disease-associated microsatellite repeat expansions.
Rohilla KJ, Gagnon KT. Rohilla KJ, et al. Acta Neuropathol Commun. 2017 Aug 29;5(1):63. doi: 10.1186/s40478-017-0468-y. Acta Neuropathol Commun. 2017. PMID: 28851463 Free PMC article. Review. - Leptomycin B alters the subcellular distribution of CRM1 (Exportin 1).
Rahmani K, Dean DA. Rahmani K, et al. Biochem Biophys Res Commun. 2017 Jun 24;488(2):253-258. doi: 10.1016/j.bbrc.2017.04.042. Epub 2017 Apr 12. Biochem Biophys Res Commun. 2017. PMID: 28412356 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous