The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA - PubMed (original) (raw)

. 1999 Jun 1;38(22):7008-18.

doi: 10.1021/bi990224y.

Affiliations

• PMID: 10353812
• DOI: 10.1021/bi990224y

The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA

S P Chandler et al. Biochemistry. 1999.

Abstract

We have investigated the interactions of the methyl-CpG binding transcriptional repressor MeCP2 with nucleosomal DNA. We find that MeCP2 forms discrete complexes with nucleosomal DNA associating with methyl-CpGs exposed in the major groove via the methyl-CpG-binding domain (MBD). In addition to the MBD, the carboxyl-terminal segment of MeCP2 facilitates binding both to naked DNA and to the nucleosome core. These observations provide a molecular mechanism by which MeCP2 can gain access to chromatin in order to target corepressor complexes that further modify chromatin structure.

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