Assembly of the paraflagellar rod and the flagellum attachment zone complex during the Trypanosoma brucei cell cycle - PubMed (original) (raw)
Assembly of the paraflagellar rod and the flagellum attachment zone complex during the Trypanosoma brucei cell cycle
L Kohl et al. J Eukaryot Microbiol. 1999 Mar-Apr.
Abstract
Trypanosomes possess a single flagellum that is attached to their cell body via the flagellum attachment zone (FAZ). The FAZ is composed of two structures: a cytoplasmic filament complex and four microtubules situated next to it. There is a complex transmembrane crosslinking of this FAZ to the paraflagellar rod (PFR) and axoneme within the flagellum. We have partially purified the FAZ complex and have produced monoclonal antibodies both against the FAZ and the paraflagellar rod. The two antibodies against the FAZ (L3B2 and L6B3) recognise the cytoplasmic filament in immunofluorescence and in immunoelectron microscopy. On western blot, they detect a doublet of high molecular weight (M(r) 200,000). Two anti-PFR antibodies (L13D6 and L8C4) recognise the paraflagellar rod in immunofluorescence, but show a difference on Western blot: L13D6 recognises both major PFR proteins, whereas L8C4 is specific for only one of them. Using these new antibodies we have shown that although the growth of both cytoplasmic FAZ filament and external PFR are related, their growth initiates at different time points during the cell cycle and the two structures elongate at distinct rates.
Similar articles
- Flagellar adhesion in Trypanosoma brucei relies on interactions between different skeletal structures in the flagellum and cell body.
Rotureau B, Blisnick T, Subota I, Julkowska D, Cayet N, Perrot S, Bastin P. Rotureau B, et al. J Cell Sci. 2014 Jan 1;127(Pt 1):204-15. doi: 10.1242/jcs.136424. Epub 2013 Oct 25. J Cell Sci. 2014. PMID: 24163437 - A repetitive protein essential for the flagellum attachment zone filament structure and function in Trypanosoma brucei.
Vaughan S, Kohl L, Ngai I, Wheeler RJ, Gull K. Vaughan S, et al. Protist. 2008 Jan;159(1):127-36. doi: 10.1016/j.protis.2007.08.005. Epub 2007 Oct 22. Protist. 2008. PMID: 17945531 - Calmodulin is required for paraflagellar rod assembly and flagellum-cell body attachment in trypanosomes.
Ginger ML, Collingridge PW, Brown RW, Sproat R, Shaw MK, Gull K. Ginger ML, et al. Protist. 2013 Jul;164(4):528-40. doi: 10.1016/j.protis.2013.05.002. Epub 2013 Jun 19. Protist. 2013. PMID: 23787017 - Assembly of the flagellum and its role in cell morphogenesis in Trypanosoma brucei.
Vaughan S. Vaughan S. Curr Opin Microbiol. 2010 Aug;13(4):453-8. doi: 10.1016/j.mib.2010.05.006. Epub 2010 Jun 10. Curr Opin Microbiol. 2010. PMID: 20541452 Review. - The Flagellum Attachment Zone: 'The Cellular Ruler' of Trypanosome Morphology.
Sunter JD, Gull K. Sunter JD, et al. Trends Parasitol. 2016 Apr;32(4):309-324. doi: 10.1016/j.pt.2015.12.010. Epub 2016 Jan 8. Trends Parasitol. 2016. PMID: 26776656 Free PMC article. Review.
Cited by
- Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes.
Link F, Borges A, Karo O, Jungblut M, Müller T, Meyer-Natus E, Krüger T, Sachs S, Jones NG, Morphew M, Sauer M, Stigloher C, McIntosh JR, Engstler M. Link F, et al. Elife. 2024 Apr 15;12:RP91194. doi: 10.7554/eLife.91194. Elife. 2024. PMID: 38619530 Free PMC article. - Spatial confinement of Trypanosoma brucei in microfluidic traps provides a new tool to study free swimming parasites.
De Niz M, Frachon E, Gobaa S, Bastin P. De Niz M, et al. PLoS One. 2023 Dec 22;18(12):e0296257. doi: 10.1371/journal.pone.0296257. eCollection 2023. PLoS One. 2023. PMID: 38134042 Free PMC article. - Implications of Flagellar Attachment Zone Proteins TcGP72 and TcFLA-1BP in Morphology, Proliferation, and Intracellular Dynamics in Trypanosoma cruzi.
Souza-Melo N, de Lima Alcantara C, Vidal JC, Rocha GM, de Souza W. Souza-Melo N, et al. Pathogens. 2023 Nov 18;12(11):1367. doi: 10.3390/pathogens12111367. Pathogens. 2023. PMID: 38003831 Free PMC article. - Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division.
Campbell PC, de Graffenried CL. Campbell PC, et al. PLoS Negl Trop Dis. 2023 Nov 2;17(11):e0011731. doi: 10.1371/journal.pntd.0011731. eCollection 2023 Nov. PLoS Negl Trop Dis. 2023. PMID: 37917723 Free PMC article. - Characterisation of TbSmee1 suggests endocytosis allows surface-bound cargo to enter the trypanosome flagellar pocket.
Schichler D, Konle A, Spath EM, Riegler S, Klein A, Seleznev A, Jung S, Wuppermann T, Wetterich N, Borges A, Meyer-Natus E, Havlicek K, Pérez Cabrera S, Niedermüller K, Sajko S, Dohn M, Malzer X, Riemer E, Tumurbaatar T, Djinovic-Carugo K, Dong G, Janzen CJ, Morriswood B. Schichler D, et al. J Cell Sci. 2023 Oct 15;136(20):jcs261548. doi: 10.1242/jcs.261548. Epub 2023 Oct 26. J Cell Sci. 2023. PMID: 37737012 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials