Molecular cloning and transmembrane structure of hCLCA2 from human lung, trachea, and mammary gland - PubMed (original) (raw)
Molecular cloning and transmembrane structure of hCLCA2 from human lung, trachea, and mammary gland
A D Gruber et al. Am J Physiol. 1999 Jun.
Abstract
The CLCA family of Ca2+-activated Cl- channels has recently been discovered, with an increasing number of closely related members isolated from different species. Here we report the cloning of the second human homolog, hCLCA2, from a human lung cDNA library. Northern blot and RT-PCR analyses revealed additional expression in trachea and mammary gland. A primary translation product of 120 kDa was cleaved into two cell surface-associated glycoproteins of 86 and 34 kDa in transfected HEK-293 cells. hCLCA2 is the first CLCA homolog for which the transmembrane structure has been systematically studied. Glycosylation site scanning and protease protection assays revealed five transmembrane domains with a large, cysteine-rich, amino-terminal extracellular domain. Whole cell patch-clamp recordings of hCLCA2-transfected HEK-293 cells detected a slightly outwardly rectifying anion conductance that was increased in the presence of the Ca2+ ionophore ionomycin and inhibited by DIDS, dithiothreitol, niflumic acid, and tamoxifen. Expression in human trachea and lung suggests that hCLCA2 may play a role in the complex pathogenesis of cystic fibrosis.
Similar articles
- Ca2+-activated Cl- channels: focus on "molecular cloning and transmembrane structure of hCLCA2 from human lung, trachea, and mammary gland".
Cuppoletti J, Malinowska DH. Cuppoletti J, et al. Am J Physiol. 1999 Jun;276(6):C1259-60. doi: 10.1152/ajpcell.1999.276.6.C1259. Am J Physiol. 1999. PMID: 10362587 Review. No abstract available. - Cloning of an epithelial chloride channel from bovine trachea.
Cunningham SA, Awayda MS, Bubien JK, Ismailov II, Arrate MP, Berdiev BK, Benos DJ, Fuller CM. Cunningham SA, et al. J Biol Chem. 1995 Dec 29;270(52):31016-26. doi: 10.1074/jbc.270.52.31016. J Biol Chem. 1995. PMID: 8537359 - Cloning and heterologous expression of a Ca2+-activated chloride channel isoform from rat brain.
Yoon IS, Jeong SM, Lee SN, Lee JH, Kim JH, Pyo MK, Lee JH, Lee BH, Choi SH, Rhim H, Choe H, Nah SY. Yoon IS, et al. Biol Pharm Bull. 2006 Nov;29(11):2168-73. doi: 10.1248/bpb.29.2168. Biol Pharm Bull. 2006. PMID: 17077509 - Molecular cloning and biochemical characterization of a truncated, secreted member of the human family of Ca2+-activated Cl- channels.
Gruber AD, Pauli BU. Gruber AD, et al. Biochim Biophys Acta. 1999 Mar 19;1444(3):418-23. doi: 10.1016/s0167-4781(99)00008-1. Biochim Biophys Acta. 1999. PMID: 10095065 - Molecular characteristics and functional diversity of CLCA family members.
Pauli BU, Abdel-Ghany M, Cheng HC, Gruber AD, Archibald HA, Elble RC. Pauli BU, et al. Clin Exp Pharmacol Physiol. 2000 Nov;27(11):901-5. doi: 10.1046/j.1440-1681.2000.03358.x. Clin Exp Pharmacol Physiol. 2000. PMID: 11071307 Review.
Cited by
- Expression of Survivin, CK7, ASH1, HMGB3, L587S, and CLCA2 in Peripheral Blood of Lung Cancer Patients by Real-Time Polymerase Chain Reaction.
Gupta S, Gupta M, Goyal B, Yadav SRM, Mirza AA, Gupta A, Rao S, Kumari K, Nanda S, Kotru M. Gupta S, et al. Cureus. 2024 Jul 12;16(7):e64386. doi: 10.7759/cureus.64386. eCollection 2024 Jul. Cureus. 2024. PMID: 39130876 Free PMC article. - Self-Cleavage of Human Chloride Channel Accessory 2 Causes a Conformational Shift That Depends on Membrane Anchorage and Is Required for Its Regulation of Store-Operated Calcium Entry.
Ramena GT, Sharma A, Chang Y, Pan Z, Elble RC. Ramena GT, et al. Biomedicines. 2023 Oct 28;11(11):2915. doi: 10.3390/biomedicines11112915. Biomedicines. 2023. PMID: 38001916 Free PMC article. - Genomic, biochemical and expressional properties reveal strong conservation of the CLCA2 gene in birds and mammals.
Bartenschlager F, Klymiuk N, Gruber AD, Mundhenk L. Bartenschlager F, et al. PeerJ. 2022 Nov 8;10:e14202. doi: 10.7717/peerj.14202. eCollection 2022. PeerJ. 2022. PMID: 36389428 Free PMC article. - Evolutionarily conserved properties of CLCA proteins 1, 3 and 4, as revealed by phylogenetic and biochemical studies in avian homologues.
Bartenschlager F, Klymiuk N, Weise C, Kuropka B, Gruber AD, Mundhenk L. Bartenschlager F, et al. PLoS One. 2022 Apr 13;17(4):e0266937. doi: 10.1371/journal.pone.0266937. eCollection 2022. PLoS One. 2022. PMID: 35417490 Free PMC article. - Epigenetic Repression of Chloride Channel Accessory 2 Transcription in Cardiac Fibroblast: Implication in Cardiac Fibrosis.
Shao T, Xue Y, Fang M. Shao T, et al. Front Cell Dev Biol. 2021 Nov 12;9:771466. doi: 10.3389/fcell.2021.771466. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34869368 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous