Permeability of single nuclear pores - PubMed (original) (raw)
Permeability of single nuclear pores
O Keminer et al. Biophys J. 1999 Jul.
Abstract
In this first application of optical single transporter recording (OSTR), a recently established technique for optically monitoring the activity of single transporters in membrane patches (Tschödrich-Rotter and Peters. 1998. J. Microsc. 192:114-125), the passive permeability of the nuclear pore complex (NPC) was measured for a homologous series of hydrophilic probe molecules. Nuclei were isolated from Xenopus oocytes and firmly attached to filters containing small cylindrical pores. Transport through membrane patches spanning filter pores was measured by scanning microphotolysis. Thus the permeability coefficients of single NPCs were determined for fluorescently labeled dextrans of approximately 4, 10, and 20 kDa. Dextrans of >/=40 kDa could not permeate the NPC. The data were consistent with a model in which the NPC contains a single diffusion channel. By application of established theories for the restricted diffusion through small pores, the diffusion channel was approximated as a cylinder with a radius of 4.4-6.1 nm (mean 5. 35 nm). Because the transport rate constant of the single NPC was known, the equivalent length of the channel could be also determined and was found to be 40-50 nm (mean 44.5 nm). The symmetry of the NPC implies that a singular component such as the diffusion channel is located at the center of the NPC. Therefore a common transport pathway apparently mediates both passive and signal-dependent transport. To test this hypothesis, measurements of signal-dependent transport and of the mutual effects signal-dependent and passive transport may exert on each other are in progress.
Similar articles
- Passive transport of macromolecules through Xenopus laevis nuclear envelope.
Enss K, Danker T, Schlune A, Buchholz I, Oberleithner H. Enss K, et al. J Membr Biol. 2003 Dec 1;196(3):147-55. doi: 10.1007/s00232-003-0632-0. J Membr Biol. 2003. PMID: 14724740 - Permeability of the nuclear envelope at isolated Xenopus oocyte nuclei studied by scanning electrochemical microscopy.
Guo J, Amemiya S. Guo J, et al. Anal Chem. 2005 Apr 1;77(7):2147-56. doi: 10.1021/ac048370j. Anal Chem. 2005. PMID: 15801749 - An optical method for recording the activity of single transporters in membrane patches.
Tschödrich-Rotter M, Peters R. Tschödrich-Rotter M, et al. J Microsc. 1998 Nov;192(Pt 2):114-25. doi: 10.1046/j.1365-2818.1998.00411.x. J Microsc. 1998. PMID: 9853370 - Nuclear transport kinetics in microarrays of nuclear envelope patches.
Peters R, Coutavas E, Siebrasse JP. Peters R, et al. J Struct Biol. 2002 Oct-Dec;140(1-3):268-78. doi: 10.1016/s1047-8477(02)00525-7. J Struct Biol. 2002. PMID: 12490174 Review. - Optical single transporter recording: transport kinetics in microarrays of membrane patches.
Peters R. Peters R. Annu Rev Biophys Biomol Struct. 2003;32:47-67. doi: 10.1146/annurev.biophys.32.110601.142429. Epub 2003 Feb 6. Annu Rev Biophys Biomol Struct. 2003. PMID: 12574067 Review.
Cited by
- Role of Transportin-SR2 in HIV-1 Nuclear Import.
Tabasi M, Nombela I, Janssens J, Lahousse AP, Christ F, Debyser Z. Tabasi M, et al. Viruses. 2021 May 4;13(5):829. doi: 10.3390/v13050829. Viruses. 2021. PMID: 34064404 Free PMC article. Review. - Cargo surface hydrophobicity is sufficient to overcome the nuclear pore complex selectivity barrier.
Naim B, Zbaida D, Dagan S, Kapon R, Reich Z. Naim B, et al. EMBO J. 2009 Sep 16;28(18):2697-705. doi: 10.1038/emboj.2009.225. Epub 2009 Aug 13. EMBO J. 2009. PMID: 19680225 Free PMC article. - Characterisation of the passive permeability barrier of nuclear pore complexes.
Mohr D, Frey S, Fischer T, Güttler T, Görlich D. Mohr D, et al. EMBO J. 2009 Sep 2;28(17):2541-53. doi: 10.1038/emboj.2009.200. Epub 2009 Aug 13. EMBO J. 2009. PMID: 19680228 Free PMC article. - Effects of multiple occupancy and interparticle interactions on selective transport through narrow channels: theory versus experiment.
Zilman A. Zilman A. Biophys J. 2009 Feb 18;96(4):1235-48. doi: 10.1016/j.bpj.2008.09.058. Biophys J. 2009. PMID: 19217844 Free PMC article. - Sterol-dependent nuclear import of ORP1S promotes LXR regulated trans-activation of apoE.
Lee S, Wang PY, Jeong Y, Mangelsdorf DJ, Anderson RG, Michaely P. Lee S, et al. Exp Cell Res. 2012 Oct 1;318(16):2128-42. doi: 10.1016/j.yexcr.2012.06.012. Epub 2012 Jun 20. Exp Cell Res. 2012. PMID: 22728266 Free PMC article.
References
- J Cell Biol. 1966 Oct;31(1):55-77 - PubMed
- Biophys J. 1996 Feb;70(2):723-32 - PubMed
- Annu Rev Biochem. 1998;67:265-306 - PubMed
- Nature. 1975 Mar 13;254(5496):109-14 - PubMed
- EMBO J. 1984 Aug;3(8):1831-6 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources