Serological diagnosis of human cysticercosis by use of recombinant antigens from Taenia solium cysticerci - PubMed (original) (raw)

Serological diagnosis of human cysticercosis by use of recombinant antigens from Taenia solium cysticerci

K Hubert et al. Clin Diagn Lab Immunol. 1999 Jul.

Abstract

A Taenia solium metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expressed as glutathione S-transferase fusion proteins and applied for serological diagnosis of human cysticercosis. An enzyme-linked immunosorbent assay was established and evaluated with 27 serum samples of La Réunion and Madagascar patients with cysticercosis. Diagnosis in these patients was established with radiological and serological procedures. For antigen NC-3 a sensitivity of 96.3% and a specificity of 91.5% for the serodiagnosis were achieved. In contrast, the sensitivity of antigen NC-9 was only 33.3%.

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Figures

FIG. 1

FIG. 1

Extinction values obtained in the NC-3 and NC-9 ELISAs with 27 serum samples from patients with cysticercosis. Symbols: ⧫, patients from La Réunion; ●, patients from Madagascar. The cutoff value, indicated by the horizontal line, was determined for both ELISAs by testing 38 negative sera. All sera were tested three times with identical results.

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