Effects of surface-coupled polyethylene oxide on human macrophage adhesion and foreign body giant cell formation in vitro - PubMed (original) (raw)
Effects of surface-coupled polyethylene oxide on human macrophage adhesion and foreign body giant cell formation in vitro
C R Jenney et al. J Biomed Mater Res. 1999 Feb.
Abstract
Surface immobilized polyethylene oxide (PEO) has been shown to efficiently reduce protein adsorption and cellular adhesion, resulting in a biologically passive surface. To explore the in vitro effects of surface immobilized PEO on the human inflammatory cells, macrophages, and foreign body giant cells (FBGCs), we developed a diisocyanate-based method for coupling PEO to amine-modified glass, a surface previously shown to enhance macrophage adhesion and FBGC formation. Contact angle analysis and X-ray photoelectron spectroscopy confirmed the presence of PEO molecules bound to the surface and revealed that PEO molecular weight significantly influenced the efficiency of PEO coupling. We used a 10-day human monocyte culture protocol to demonstrate that the presence of surface coupled PEO molecules does not significantly decrease initial monocyte density or monocyte-derived macrophage density after 3 days. However, PEO-coupled surfaces significantly reduced long-term monocyte-derived macrophage density and virtually eliminated interleukin-4-induced FBGC formation observed at day 10. The cellular response to these PEO-coupled surfaces was related to the molecular weight of the PEO chains, which was varied between 200 Da and 18.5 kDa. These results suggest that an optimized PEO surface treatment may be effective in reducing inflammatory cell adhesion and possible degradation during the inflammatory response to an implanted biomedical device.
Copyright 1999 John Wiley & Sons, Inc.
Similar articles
- Human monocyte/macrophage adhesion, macrophage motility, and IL-4-induced foreign body giant cell formation on silane-modified surfaces in vitro. Student Research Award in the Master's Degree Candidate Category, 24th Annual Meeting of the Society for Biomaterials, San Diego, CA, April 22-26, 1998.
Jenney CR, DeFife KM, Colton E, Anderson JM. Jenney CR, et al. J Biomed Mater Res. 1998 Aug;41(2):171-84. doi: 10.1002/(sici)1097-4636(199808)41:2<171::aid-jbm1>3.0.co;2-f. J Biomed Mater Res. 1998. PMID: 9638521 - Alkylsilane-modified surfaces: inhibition of human macrophage adhesion and foreign body giant cell formation.
Jenney CR, Anderson JM. Jenney CR, et al. J Biomed Mater Res. 1999 Jul;46(1):11-21. doi: 10.1002/(sici)1097-4636(199907)46:1<11::aid-jbm2>3.0.co;2-y. J Biomed Mater Res. 1999. PMID: 10357131 - Adsorbed serum proteins responsible for surface dependent human macrophage behavior.
Jenney CR, Anderson JM. Jenney CR, et al. J Biomed Mater Res. 2000 Mar 15;49(4):435-47. doi: 10.1002/(sici)1097-4636(20000315)49:4<435::aid-jbm2>3.0.co;2-y. J Biomed Mater Res. 2000. PMID: 10602077 - Foreign body reaction to biomaterials.
Anderson JM, Rodriguez A, Chang DT. Anderson JM, et al. Semin Immunol. 2008 Apr;20(2):86-100. doi: 10.1016/j.smim.2007.11.004. Epub 2007 Dec 26. Semin Immunol. 2008. PMID: 18162407 Free PMC article. Review. - Multinucleated giant cells.
Anderson JM. Anderson JM. Curr Opin Hematol. 2000 Jan;7(1):40-7. doi: 10.1097/00062752-200001000-00008. Curr Opin Hematol. 2000. PMID: 10608503 Review.
Cited by
- Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion.
Faust JJ, Christenson W, Doudrick K, Heddleston J, Chew TL, Lampe M, Balabiyev A, Ros R, Ugarova TP. Faust JJ, et al. J Vis Exp. 2018 Mar 14;(133):56866. doi: 10.3791/56866. J Vis Exp. 2018. PMID: 29608169 Free PMC article. - Macrophages, Foreign Body Giant Cells and Their Response to Implantable Biomaterials.
Sheikh Z, Brooks PJ, Barzilay O, Fine N, Glogauer M. Sheikh Z, et al. Materials (Basel). 2015 Aug 28;8(9):5671-5701. doi: 10.3390/ma8095269. Materials (Basel). 2015. PMID: 28793529 Free PMC article. Review. - Development of fusogenic glass surfaces that impart spatiotemporal control over macrophage fusion: Direct visualization of multinucleated giant cell formation.
Faust JJ, Christenson W, Doudrick K, Ros R, Ugarova TP. Faust JJ, et al. Biomaterials. 2017 Jun;128:160-171. doi: 10.1016/j.biomaterials.2017.02.031. Epub 2017 Mar 2. Biomaterials. 2017. PMID: 28340410 Free PMC article. - The use of CD47-modified biomaterials to mitigate the immune response.
Tengood JE, Levy RJ, Stachelek SJ. Tengood JE, et al. Exp Biol Med (Maywood). 2016 May;241(10):1033-41. doi: 10.1177/1535370216647130. Epub 2016 May 10. Exp Biol Med (Maywood). 2016. PMID: 27190273 Free PMC article. Review. - Modification of biomaterials with a self-protein inhibits the macrophage response.
Kim YK, Que R, Wang SW, Liu WF. Kim YK, et al. Adv Healthc Mater. 2014 Jul;3(7):989-94. doi: 10.1002/adhm.201300532. Epub 2014 Feb 20. Adv Healthc Mater. 2014. PMID: 24573988 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical