Prototypal diarrheagenic strains of Hafnia alvei are actually members of the genus Escherichia - PubMed (original) (raw)

Prototypal diarrheagenic strains of Hafnia alvei are actually members of the genus Escherichia

J M Janda et al. J Clin Microbiol. 1999 Aug.

Abstract

We analyzed five bacterial strains, designated 19982, 9194, 10457, 10790, and 12502, that were isolated from stool specimens of individuals with diarrheal illness by the International Centre for Diarrhoeal Disease Research in Dhaka, Bangladesh (M. J. Albert, S. M. Faruque, M. Ansaruzzaman, M. M. Islam, K. Haider, K. Alam, I. Kabir, and R. Robins-Browne, J. Med. Microbiol. 37:310-314, 1992). The strains were initially identified as Hafnia alvei with a commercial identification system and were reported to contain the eae gene of enteropathogenic Escherichia coli. Results of conventional biochemical analyses, testing of susceptibility to cephalothin, lysis by a Hafnia-specific phage, and amplification of the outer membrane protein gene phoE with species-specific primers support the identification of these strains as members of the genus Escherichia rather than Hafnia alvei. These strains varied from typical E. coli strains by their inability to produce acid from lactose or D-sorbitol and failure to elaborate the enzyme beta-D-glucuronidase. PCR analysis confirmed previous findings that the strains were positive for the eae gene and negative for other virulence markers present among recognized categories of diarrheagenic E. coli. Our findings support the hypothesis that these strains are a new category of diarrheagenic isolates belonging to the genus Escherichia and illustrate the importance of using multiple methodologies when identifying new bacterial agents of diarrheal disease.

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Figures

FIG. 1

FIG. 1

PFGE profile (_Xba_I) of Albert’s strains and reference strains of H. alvei. Lanes 1 and 9, bacteriophage lambda markers; lanes 2, Albert’s strain 12502; lane 3, Albert’s strain 10790; lane 4, Albert’s strain 10457; lane 5, Albert’s strain 9194; lane 6, Albert’s strain 19982; lane 7, H. alvei ATCC 13337; and lane 8, H. alvei ATCC 29927.

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References

    1. Abbott S L, Janda J M. Bacterial gastroenteritis. I. Incidence and etiologic agents. Clin Microbiol Newsl. 1992;14:17–21.
    1. Albert M J, Alam K, Islam M, Montanaro J, Rahman A S M H, Haider K, Hossain M A, Kibriya A K M G, Tzipori S. Hafnia alvei, a probable cause of diarrhea in humans. Infect Immun. 1991;59:1507–1513. - PMC - PubMed
    1. Albert M J, Faruque S M, Ansaruzzaman M, Islam M M, Haider K, Alam K, Kabir I, Robins-Browne R. Sharing of virulence-associated properties at the phenotypic and genetic levels between enteropathogenic Escherichia coli and Hafnia alvei. J Med Microbiol. 1992;37:310–314. - PubMed
    1. Brenner D J. Phylogenetic classification of bacteria and recent developments in bacterial classification and nomenclature: impact on clinical microbiology. Clin Microbiol Newsl. 1988;10:153–156.
    1. Cebula T A, Payne W L, Feng P. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J Clin Microbiol. 1995;32:248–250. - PMC - PubMed

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