The steady-state turnover of murine epidermal Langerhans cells - PubMed (original) (raw)
The steady-state turnover of murine epidermal Langerhans cells
M Ghaznawie et al. Br J Dermatol. 1999 Jul.
Abstract
We have investigated the steady-state turnover of murine epidermal Langerhans cells (LCs) using an X-irradiation model, 3H-thymidine autoradiography and cultured epidermal sheet explants, and by assessing the LC population in normal mice. The LC density after whole-body irradiation without any cutaneous shielding was not significantly different from that in skin shielded during whole-body irradiation (P > 0.05), indicating that the additional irradiation to the skin did not contribute to a decrease in LC density. In both instances, the LC number gradually decreased in a linear fashion. The results indicate that epidermal LCs continuously leave the epidermis and are continually replaced by circulating precursor cells from the bone marrow at a steady rate. Autoradiographic studies after a pulse injection of 3H-thymidine showed a labelling index of 0.013%, indicating that local mitosis is not an important contributor to the maintenance of the epidermal LC population. Although local X-irradiation resulted in temporary reduction of LC density, epidermal sheet explant culture obtained immediately after local X-irradiation showed no difference in LC density as compared with control unirradiated skin, indicating that the decrease in LC density was not due to significant LC destruction. From these data, we calculated that the half-life of murine LCs in the epidermis is approximately 9 days.
Similar articles
- Labelling of murine epidermal Langerhans cells with H3-thymidine.
Mackenzie IC. Mackenzie IC. Am J Anat. 1975 Oct;144(2):127-36. doi: 10.1002/aja.1001440202. Am J Anat. 1975. PMID: 126643 - Regulation of immune activity by mild (fever-range) whole body hyperthermia: effects on epidermal Langerhans cells.
Ostberg JR, Patel R, Repasky EA. Ostberg JR, et al. Cell Stress Chaperones. 2000 Nov;5(5):458-61. doi: 10.1379/1466-1268(2000)005<0458:roiabm>2.0.co;2. Cell Stress Chaperones. 2000. PMID: 11189452 Free PMC article. - Establishing and maintaining the Langerhans cell network.
Chopin M, Nutt SL. Chopin M, et al. Semin Cell Dev Biol. 2015 May;41:23-9. doi: 10.1016/j.semcdb.2014.02.001. Epub 2014 Feb 7. Semin Cell Dev Biol. 2015. PMID: 24513231 Review. - Epidermal cell cultures as models for living epidermis.
Prunieras M. Prunieras M. J Invest Dermatol. 1979 Aug;73(2):135-7. doi: 10.1111/1523-1747.ep12556751. J Invest Dermatol. 1979. PMID: 379239 Review. No abstract available.
Cited by
- Langerhans cells renew in the skin throughout life under steady-state conditions.
Merad M, Manz MG, Karsunky H, Wagers A, Peters W, Charo I, Weissman IL, Cyster JG, Engleman EG. Merad M, et al. Nat Immunol. 2002 Dec;3(12):1135-41. doi: 10.1038/ni852. Epub 2002 Nov 4. Nat Immunol. 2002. PMID: 12415265 Free PMC article. - Dendritic cells, viruses, and the development of atopic disease.
Tam JS, Grayson MH. Tam JS, et al. J Allergy (Cairo). 2012;2012:936870. doi: 10.1155/2012/936870. Epub 2012 Oct 15. J Allergy (Cairo). 2012. PMID: 23118777 Free PMC article. - Emerging role of dendritic cells in respiratory viral infection.
Grayson MH, Holtzman MJ. Grayson MH, et al. J Mol Med (Berl). 2007 Oct;85(10):1057-68. doi: 10.1007/s00109-007-0212-3. Epub 2007 May 22. J Mol Med (Berl). 2007. PMID: 17891367 Review. - Langerhans cells and more: langerin-expressing dendritic cell subsets in the skin.
Romani N, Clausen BE, Stoitzner P. Romani N, et al. Immunol Rev. 2010 Mar;234(1):120-41. doi: 10.1111/j.0105-2896.2009.00886.x. Immunol Rev. 2010. PMID: 20193016 Free PMC article. Review. - Depletion of Langerhans cells in human papillomavirus type 16-infected skin is associated with E6-mediated down regulation of E-cadherin.
Matthews K, Leong CM, Baxter L, Inglis E, Yun K, Bäckström BT, Doorbar J, Hibma M. Matthews K, et al. J Virol. 2003 Aug;77(15):8378-85. doi: 10.1128/jvi.77.15.8378-8385.2003. J Virol. 2003. PMID: 12857907 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical