Electron spin resonance characterization of liquid ordered phase of detergent-resistant membranes from RBL-2H3 cells - PubMed (original) (raw)

Comparative Study

Electron spin resonance characterization of liquid ordered phase of detergent-resistant membranes from RBL-2H3 cells

M Ge et al. Biophys J. 1999 Aug.

Abstract

The dynamic structure of detergent-resistant membranes (DRMs) isolated from RBL-2H3 cells was characterized using two different acyl chain spin-labeled phospholipids (5PC and 16PC), a headgroup labeled sphingomyelin (SM) analog (SD-Tempo) and a spin-labeled cholestane (CSL). It was shown, by comparison to dispersions of SM, dipalmitoylphosphatidylcholine (DPPC), and DPPC/cholesterol of molar ratio 1, that DRM contains a substantial amount of liquid ordered phase: 1) The rotational diffusion rates (R( perpendicular)) of 16PC in DRM between -5 degrees C and 45 degrees C are nearly the same as those in molar ratio DPPC/Chol = 1 dispersions, and they are substantially greater than R( perpendicular) in pure DPPC dispersions in the gel phase studied above 20 degrees C; 2) The order parameters (S) of 16PC in DRM at temperatures above 4 degrees C are comparable to those in DPPC/Chol = 1 dispersions, but are greater than those in DPPC dispersions in both the gel and liquid crystalline phases. 3) Similarly, R( perpendicular) for 5PC and CSL in DRM is greater than in pure SM dispersions in the gel phase, and S for these labels in DRM is greater than in the SM dispersions in both the gel and liquid crystalline phases. 4) R( perpendicular) of SD-Tempo in DRM is greater than in dispersions of SM in both gel and liquid phases, consistent with the liquid-like mobility in the acyl chain region in DRM. However, S of SD-Tempo in DRM is substantially less than that of this spin label in SM in gel and liquid crystalline phases (in absolute values), indicating that the headgroup region in DRMs is less ordered than in pure SM. These results support the hypothesis that plasma membranes contain DRM domains with a liquid ordered phase that may coexist with a liquid crystalline phase. There also appears to be a coexisting region in DRMs in which the chain labels 16PC and 5PC are found to cluster. We suggest that other biological membranes containing high concentrations of cholesterol also contain a liquid ordered phase.

PubMed Disclaimer

Similar articles

• A solid-state NMR study of phospholipid-cholesterol interactions: sphingomyelin-cholesterol binary systems.
  Guo W, Kurze V, Huber T, Afdhal NH, Beyer K, Hamilton JA. Guo W, et al. Biophys J. 2002 Sep;83(3):1465-78. doi: 10.1016/S0006-3495(02)73917-9. Biophys J. 2002. PMID: 12202372 Free PMC article.
• Structure, composition, and peptide binding properties of detergent soluble bilayers and detergent resistant rafts.
  Gandhavadi M, Allende D, Vidal A, Simon SA, McIntosh TJ. Gandhavadi M, et al. Biophys J. 2002 Mar;82(3):1469-82. doi: 10.1016/S0006-3495(02)75501-X. Biophys J. 2002. PMID: 11867462 Free PMC article.
• Structure of detergent-resistant membrane domains: does phase separation occur in biological membranes?
  Brown DA, London E. Brown DA, et al. Biochem Biophys Res Commun. 1997 Nov 7;240(1):1-7. doi: 10.1006/bbrc.1997.7575. Biochem Biophys Res Commun. 1997. PMID: 9367871 Review.
• Properties of Langmuir and solid supported lipid films with sphingomyelin.
  Jurak M, Golabek M, Holysz L, Chibowski E. Jurak M, et al. Adv Colloid Interface Sci. 2015 Aug;222:385-97. doi: 10.1016/j.cis.2014.03.008. Epub 2014 Mar 28. Adv Colloid Interface Sci. 2015. PMID: 24725646 Review.

Cited by

• Phase-separation and domain-formation in cholesterol-sphingomyelin mixture: pulse-EPR oxygen probing.
  Mainali L, Raguz M, Subczynski WK. Mainali L, et al. Biophys J. 2011 Aug 17;101(4):837-46. doi: 10.1016/j.bpj.2011.07.014. Biophys J. 2011. PMID: 21843474 Free PMC article.
• Protective role of the dynamin inhibitor Dynasore against the cholesterol-dependent cytolysin of Trueperella pyogenes.
  Preta G, Lotti V, Cronin JG, Sheldon IM. Preta G, et al. FASEB J. 2015 Apr;29(4):1516-28. doi: 10.1096/fj.14-265207. Epub 2014 Dec 30. FASEB J. 2015. PMID: 25550455 Free PMC article.
• Regulating Lipid Composition Rationalizes Acyl Tail Saturation Homeostasis in Ectotherms.
  Girard M, Bereau T. Girard M, et al. Biophys J. 2020 Sep 1;119(5):892-899. doi: 10.1016/j.bpj.2020.07.024. Epub 2020 Aug 6. Biophys J. 2020. PMID: 32814063 Free PMC article.
• Membrane order and molecular dynamics associated with IgE receptor cross-linking in mast cells.
  Davey AM, Walvick RP, Liu Y, Heikal AA, Sheets ED. Davey AM, et al. Biophys J. 2007 Jan 1;92(1):343-55. doi: 10.1529/biophysj.106.088815. Epub 2006 Oct 13. Biophys J. 2007. PMID: 17040981 Free PMC article.
• Binding of cross-linked glycosylphosphatidylinositol-anchored proteins to discrete actin-associated sites and cholesterol-dependent domains.
  Suzuki K, Sheetz MP. Suzuki K, et al. Biophys J. 2001 Oct;81(4):2181-9. doi: 10.1016/S0006-3495(01)75866-3. Biophys J. 2001. PMID: 11566789 Free PMC article.

References

1. 1. Biochim Biophys Acta. 1978 Oct 19;513(1):43-58 - PubMed
2. 1. Biochemistry. 1977 Oct 4;16(20):4344-9 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1979 Jan;76(1):15-8 - PubMed
4. 1. Biochemistry. 1979 Jul 24;18(15):3280-5 - PubMed
5. 1. Biochim Biophys Acta. 1982 Mar 23;686(1):137-40 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Europe PubMed Central
  • PubMed Central