CENP-C is necessary but not sufficient to induce formation of a functional centromere - PubMed (original) (raw)
CENP-C is necessary but not sufficient to induce formation of a functional centromere
T Fukagawa et al. EMBO J. 1999.
Abstract
CENP-C is an evolutionarily conserved centromeric protein. We have used the chicken DT40 cell line to test the idea that CENP-C is sufficient as well as necessary for the formation of a functional centromere. We have compared the effects of disrupting the localization of CENP-C with those of inducibly overexpressing the protein. Removing CENP-C from the centromere causes disassembly of the centromere protein complex and blocks cells at the metaphase-anaphase junction. Overexpressed CENP-C is associated with an increase in errors of chromosome segregation and inhibits the completion of mitosis. However, the excess CENP-C does not disrupt the native centromeres detectably and does not associate with another conserved centromere protein, ZW10. The distribution of the excess CENP-C changes during the cell cycle. In metaphase, the excess CENP-C coats the chromosome arms. At the metaphase-anaphase transition, the excess CENP-C clusters, and during interphase it is present in large bodies which form around pre-existing centromeres which are also clustered. These results indicate that CENP-C is necessary but not sufficient for the formation of a functional centromere and suggest that the structure of CENP-C may be regulated during the cell cycle.
Similar articles
- Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase.
Schuh M, Lehner CF, Heidmann S. Schuh M, et al. Curr Biol. 2007 Feb 6;17(3):237-43. doi: 10.1016/j.cub.2006.11.051. Epub 2007 Jan 11. Curr Biol. 2007. PMID: 17222555 - Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway.
Goshima G, Kiyomitsu T, Yoda K, Yanagida M. Goshima G, et al. J Cell Biol. 2003 Jan 6;160(1):25-39. doi: 10.1083/jcb.200210005. Epub 2003 Jan 6. J Cell Biol. 2003. PMID: 12515822 Free PMC article. - CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA.
Ohzeki J, Nakano M, Okada T, Masumoto H. Ohzeki J, et al. J Cell Biol. 2002 Dec 9;159(5):765-75. doi: 10.1083/jcb.200207112. Epub 2002 Dec 2. J Cell Biol. 2002. PMID: 12460987 Free PMC article. - Two distinct pathways responsible for the loading of CENP-A to centromeres in the fission yeast cell cycle.
Takahashi K, Takayama Y, Masuda F, Kobayashi Y, Saitoh S. Takahashi K, et al. Philos Trans R Soc Lond B Biol Sci. 2005 Mar 29;360(1455):595-606; discussion 606-7. doi: 10.1098/rstb.2004.1614. Philos Trans R Soc Lond B Biol Sci. 2005. PMID: 15897182 Free PMC article. Review. - The centromere: kinetochore complex.
Vig BK. Vig BK. Southeast Asian J Trop Med Public Health. 1995;26 Suppl 1:68-76. Southeast Asian J Trop Med Public Health. 1995. PMID: 8629145 Review.
Cited by
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.
Jeffery D, Lochhead M, Almouzni G. Jeffery D, et al. Results Probl Cell Differ. 2022;70:221-261. doi: 10.1007/978-3-031-06573-6_7. Results Probl Cell Differ. 2022. PMID: 36348109 - Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions.
Takenoshita Y, Hara M, Fukagawa T. Takenoshita Y, et al. Nat Commun. 2022 Feb 14;13(1):851. doi: 10.1038/s41467-022-28403-8. Nat Commun. 2022. PMID: 35165266 Free PMC article. - Mobility of kinetochore proteins measured by FRAP analysis in living cells.
Watanabe R, Hirano Y, Hara M, Hiraoka Y, Fukagawa T. Watanabe R, et al. Chromosome Res. 2022 Mar;30(1):43-57. doi: 10.1007/s10577-021-09678-x. Epub 2022 Jan 8. Chromosome Res. 2022. PMID: 34997387 Free PMC article. - Cryo-EM structure of the CENP-A nucleosome in complex with phosphorylated CENP-C.
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T. Ariyoshi M, et al. EMBO J. 2021 Mar 1;40(5):e105671. doi: 10.15252/embj.2020105671. Epub 2021 Jan 19. EMBO J. 2021. PMID: 33463726 Free PMC article. - The centromere comes into focus: from CENP-A nucleosomes to kinetochore connections with the spindle.
Kixmoeller K, Allu PK, Black BE. Kixmoeller K, et al. Open Biol. 2020 Jun;10(6):200051. doi: 10.1098/rsob.200051. Epub 2020 Jun 10. Open Biol. 2020. PMID: 32516549 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous