A role for p38(MAPK)/HSP27 pathway in smooth muscle cell migration - PubMed (original) (raw)
. 1999 Aug 20;274(34):24211-9.
doi: 10.1074/jbc.274.34.24211.
Affiliations
- PMID: 10446196
- DOI: 10.1074/jbc.274.34.24211
Free article
A role for p38(MAPK)/HSP27 pathway in smooth muscle cell migration
J C Hedges et al. J Biol Chem. 1999.
Free article
Abstract
Smooth muscle cells are exposed to growth factors and cytokines that contribute to pathological states including airway hyperresponsiveness, atherosclerosis, angiogenesis, smooth muscle hypertrophy, and hyperplasia. A common feature of several of these conditions is migration of smooth muscle beyond the initial boundary of the organ. Signal transduction pathways activated by extracellular signals that instigate migration are mostly undefined in smooth muscles. We measured migration of cultured tracheal myocytes in response to platelet-derived growth factor, interleukin-1beta, and transforming growth factor-beta. Cellular migration was blocked by SB203580, an inhibitor of p38(MAPK). Time course experiments demonstrated increased phosphorylation of p38(MAPK). Activation of p38(MAPK) resulted in the phosphorylation of HSP27 (heat shock protein 27), which may modulate F-actin polymerization. Inhibition of p38(MAPK) activity inhibited phosphorylation of HSP27. Adenovirus-mediated expression of activated mutant MAPK kinase 6b(E), an upstream activator for p38(MAPK), increased cell migration, whereas overexpression of p38alpha MAPK dominant negative mutant and an HSP27 phosphorylation mutant blocked cell migration completely. The results indicate that activation of the p38(MAPK) pathway by growth factors and proinflammatory cytokines regulates smooth muscle cell migration and may contribute to pathological states involving smooth muscle dysfunction.
Similar articles
- p21-activated kinase 1 participates in tracheal smooth muscle cell migration by signaling to p38 Mapk.
Dechert MA, Holder JM, Gerthoffer WT. Dechert MA, et al. Am J Physiol Cell Physiol. 2001 Jul;281(1):C123-32. doi: 10.1152/ajpcell.2001.281.1.C123. Am J Physiol Cell Physiol. 2001. PMID: 11401834 - Phosphorylation of the 27-kDa heat shock protein via p38 MAP kinase and MAPKAP kinase in smooth muscle.
Larsen JK, Yamboliev IA, Weber LA, Gerthoffer WT. Larsen JK, et al. Am J Physiol. 1997 Nov;273(5):L930-40. doi: 10.1152/ajplung.1997.273.5.L930. Am J Physiol. 1997. PMID: 9374719 - Syk contributes to PDGF-BB-mediated migration of rat aortic smooth muscle cells via MAPK pathways.
Lee CK, Lee HM, Kim HJ, Park HJ, Won KJ, Roh HY, Choi WS, Jeon BH, Park TK, Kim B. Lee CK, et al. Cardiovasc Res. 2007 Apr 1;74(1):159-68. doi: 10.1016/j.cardiores.2007.01.012. Epub 2007 Jan 18. Cardiovasc Res. 2007. PMID: 17303097 - Modulation of actin dynamics during stress and physiological stimulation by a signaling pathway involving p38 MAP kinase and heat-shock protein 27.
Landry J, Huot J. Landry J, et al. Biochem Cell Biol. 1995 Sep-Oct;73(9-10):703-7. doi: 10.1139/o95-078. Biochem Cell Biol. 1995. PMID: 8714691 Review. - Migration of airway smooth muscle cells.
Gerthoffer WT. Gerthoffer WT. Proc Am Thorac Soc. 2008 Jan 1;5(1):97-105. doi: 10.1513/pats.200704-051VS. Proc Am Thorac Soc. 2008. PMID: 18094091 Free PMC article. Review.
Cited by
- STEAP2 promotes hepatocellular carcinoma progression via increased copper levels and stress-activated MAP kinase activity.
Torrez CZ, Easley A, Bouamar H, Zheng G, Gu X, Yang J, Chiu YC, Chen Y, Halff GA, Cigarroa FG, Sun LZ. Torrez CZ, et al. Sci Rep. 2024 Jun 3;14(1):12753. doi: 10.1038/s41598-024-63368-2. Sci Rep. 2024. PMID: 38830975 Free PMC article. - Kisspeptin/KISS1R Signaling Modulates Human Airway Smooth Muscle Cell Migration.
Balraj P, Ambhore NS, Ramakrishnan YS, Borkar NA, Banerjee P, Reza MI, Varadharajan S, Kumar A, Pabelick CM, Prakash YS, Sathish V. Balraj P, et al. Am J Respir Cell Mol Biol. 2024 Jun;70(6):507-518. doi: 10.1165/rcmb.2023-0469OC. Am J Respir Cell Mol Biol. 2024. PMID: 38512807 - Revisiting p38 Mitogen-Activated Protein Kinases (MAPK) in Inflammatory Arthritis: A Narrative of the Emergence of MAPK-Activated Protein Kinase Inhibitors (MK2i).
Ganguly P, Macleod T, Wong C, Harland M, McGonagle D. Ganguly P, et al. Pharmaceuticals (Basel). 2023 Sep 12;16(9):1286. doi: 10.3390/ph16091286. Pharmaceuticals (Basel). 2023. PMID: 37765094 Free PMC article. Review. - Targeting Microtubule-Associated Protein Tau in Chemotherapy-Resistant Models of High-Grade Serous Ovarian Carcinoma.
Barbolina MV. Barbolina MV. Cancers (Basel). 2022 Sep 19;14(18):4535. doi: 10.3390/cancers14184535. Cancers (Basel). 2022. PMID: 36139693 Free PMC article. - Bronchial smooth muscle cell in asthma: where does it fit?
Hassoun D, Rose L, Blanc FX, Magnan A, Loirand G, Sauzeau V. Hassoun D, et al. BMJ Open Respir Res. 2022 Sep;9(1):e001351. doi: 10.1136/bmjresp-2022-001351. BMJ Open Respir Res. 2022. PMID: 36109087 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous