Molecular mechanism for the spontaneous generation of pigmentless Porphyromonas gingivalis mutants - PubMed (original) (raw)

FIG. 2

Genomic maps of the proposed orientations of the hagA and kgp genes of P. gingivalis 381. The major restriction sites of hagA and kgp are indicated. Restriction sites: K, _Kpn_I (nucleotides 3760, 5116, 6484, 7852, 10606, 12589, and 16087); B, _Bam_HI (nucleotides 3309, 3672, 12484, and 15999); N, _Nco_I (nucleotides 4840, 6208, 7576, 8944, 17170, and 18643); S, _Sma_I (nucleotides 4583, 5951, 7319, 8687, 16913, 19245, and 19554); V, _Van_91I (nucleotides 1741 and 20629). Solid boxes on the map show the probes N (for the Kgp N-terminal protease domain), C (for the Kgp adhesin domain), _hagA_N (for the exact HagA N-terminal region), and _kgp_C (for the exact Kgp C-terminal region). Boxes with hatching labeled “_hagA_” and “kgp” represent the adhesin regions of the hagA and kgp genes, respectively. The stippled box represents the N-terminal domain and protease domain of Kgp. The boxes above the restriction enzyme map with hatching, vertical lines, and shading represent the proposed minimal deleted regions of WK-W, MT10-W, and G102-W, respectively.