Continued RAG expression in late stages of B cell development and no apparent re-induction after immunization - PubMed (original) (raw)
. 1999 Aug 12;400(6745):682-7.
doi: 10.1038/23287.
Affiliations
- PMID: 10458165
- DOI: 10.1038/23287
Continued RAG expression in late stages of B cell development and no apparent re-induction after immunization
W Yu et al. Nature. 1999.
Abstract
Models of B-cell development in the immune system suggest that only those immature B cells in the bone marrow that undergo receptor editing express V(D)J-recombination-activating genes (RAGs). Here we investigate the regulation of RAG expression in transgenic mice carrying a bacterial artificial chromosome that encodes a green fluorescent protein reporter instead of RAG2. We find that the reporter is expressed in all immature B cells in the bone marrow and spleen. Endogenous RAG messenger RNA is expressed in immature B cells in bone marrow and spleen and decreases by two orders of magnitude as they acquire higher levels of surface immunoglobulin M (IgM). Once RAG expression is stopped it is not re-induced during immune responses. Our findings may help to reconcile a series of apparently contradictory observations, and suggest a new model for the mechanisms that regulate allelic exclusion, receptor editing and tolerance.
Comment in
- Developing B-cell theories.
Schatz DG. Schatz DG. Nature. 1999 Aug 12;400(6745):614-5, 617. doi: 10.1038/23134. Nature. 1999. PMID: 10458155 No abstract available.
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