GTP hydrolysis links initiation and termination of nuclear import on the nucleoporin nup358 - PubMed (original) (raw)
. 1999 Sep 10;274(37):26493-502.
doi: 10.1074/jbc.274.37.26493.
Affiliations
- PMID: 10473610
- DOI: 10.1074/jbc.274.37.26493
Free article
GTP hydrolysis links initiation and termination of nuclear import on the nucleoporin nup358
N R Yaseen et al. J Biol Chem. 1999.
Free article
Abstract
Binding of GTP-bound Ran (RanGTP) to karyopherin beta1 (Kapbeta1) releases import cargo into the nucleus. Using an ultrastructural, biochemical, and functional approach, we have studied the mechanism by which Kapbeta1.RanGTP is recycled at the nuclear pore complex for repeated rounds of import. In vitro, Kapbeta1 bound to the RanBP1-homologous (RBH) domains of Nup358 in the presence of either RanGTP or RanGDP, forming trimeric complexes. The Kapbeta1.RanGTP. RBH complex resisted dissociation by RanBP1 and GTP hydrolysis by Ran GTPase activating protein 1. Ran-dependent binding of gold-conjugated Kapbeta1 to the cytoplasmic fibers of the nuclear pore complex in digitonin-permeabilized cells and RanBP1 competition confirmed the in vitro binding data. Interaction of karyopherin alpha and a classical nuclear localization sequence peptide with the Kapbeta1.RanGTP.RBH complex stimulated GTP hydrolysis by Ran GTPase activating protein 1 both in vitro and in permeabilized cells. This GTP hydrolysis was required for reinitiation of import of a nuclear localization sequence-bearing substrate in permeabilized cells. These data suggest that GTP hydrolysis on the RBH domains of Nup358 couples the termination of one cycle of nuclear import with the initiation of the next.
Similar articles
- RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import.
Chi NC, Adam EJ, Visser GD, Adam SA. Chi NC, et al. J Cell Biol. 1996 Nov;135(3):559-69. doi: 10.1083/jcb.135.3.559. J Cell Biol. 1996. PMID: 8909533 Free PMC article. - Two distinct classes of Ran-binding sites on the nucleoporin Nup-358.
Yaseen NR, Blobel G. Yaseen NR, et al. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5516-21. doi: 10.1073/pnas.96.10.5516. Proc Natl Acad Sci U S A. 1999. PMID: 10318915 Free PMC article. - Identification of different roles for RanGDP and RanGTP in nuclear protein import.
Görlich D, Panté N, Kutay U, Aebi U, Bischoff FR. Görlich D, et al. EMBO J. 1996 Oct 15;15(20):5584-94. EMBO J. 1996. PMID: 8896452 Free PMC article. - Distinct nuclear import and export pathways mediated by members of the karyopherin beta family.
Moroianu J. Moroianu J. J Cell Biochem. 1998 Aug 1;70(2):231-9. J Cell Biochem. 1998. PMID: 9671229 Review. - Molecular mechanisms of nuclear protein transport.
Moroianu J. Moroianu J. Crit Rev Eukaryot Gene Expr. 1997;7(1-2):61-72. doi: 10.1615/critreveukargeneexpr.v7.i1-2.40. Crit Rev Eukaryot Gene Expr. 1997. PMID: 9034715 Review.
Cited by
- The oncogene eIF4E reprograms the nuclear pore complex to promote mRNA export and oncogenic transformation.
Culjkovic-Kraljacic B, Baguet A, Volpon L, Amri A, Borden KL. Culjkovic-Kraljacic B, et al. Cell Rep. 2012 Aug 30;2(2):207-15. doi: 10.1016/j.celrep.2012.07.007. Epub 2012 Aug 16. Cell Rep. 2012. PMID: 22902403 Free PMC article. - TorsinA dysfunction causes persistent neuronal nuclear pore defects.
Pappas SS, Liang CC, Kim S, Rivera CO, Dauer WT. Pappas SS, et al. Hum Mol Genet. 2018 Feb 1;27(3):407-420. doi: 10.1093/hmg/ddx405. Hum Mol Genet. 2018. PMID: 29186574 Free PMC article. - Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores.
Roscioli E, Di Francesco L, Bolognesi A, Giubettini M, Orlando S, Harel A, Schininà ME, Lavia P. Roscioli E, et al. J Cell Biol. 2012 Feb 20;196(4):435-50. doi: 10.1083/jcb.201109104. Epub 2012 Feb 13. J Cell Biol. 2012. PMID: 22331847 Free PMC article. - The nucleoporin Nup98 associates with the intranuclear filamentous protein network of TPR.
Fontoura BM, Dales S, Blobel G, Zhong H. Fontoura BM, et al. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3208-13. doi: 10.1073/pnas.061014698. Proc Natl Acad Sci U S A. 2001. PMID: 11248057 Free PMC article. - Emerging roles for nucleoporins in reproductive cellular physiology 1.
Preston CC, Storm EC, Leonard RJ, Faustino RS. Preston CC, et al. Can J Physiol Pharmacol. 2019 Apr;97(4):257-264. doi: 10.1139/cjpp-2018-0436. Epub 2018 Nov 2. Can J Physiol Pharmacol. 2019. PMID: 30388388 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous