Characterisation of immune mediator release during the immediate response to segmental mucosal challenge in the jejunum of patients with food allergy - PubMed (original) (raw)

Characterisation of immune mediator release during the immediate response to segmental mucosal challenge in the jejunum of patients with food allergy

J Santos et al. Gut. 1999 Oct.

Abstract

Background: Food allergy is a common complaint among patients with a broad spectrum of abdominal and extra-abdominal symptoms that must be distinguished from other more common non-immunological food intolerances.

Aims: To investigate whether human intestinal hypersensitivity reactions are associated with detectable release of inflammatory mediators from activated cells, which may serve as a biological marker of true allergic reactions.

Patients/methods: In eight patients with food allergy and seven healthy volunteers, a closed-segment perfusion technique was used to investigate the effects of jejunal food challenge on luminal release of tryptase, histamine, prostaglandin D(2), eosinophil cationic protein, peroxidase activity, and water flux.

Results: Intraluminal administration of food antigens induced a rapid increase in intestinal release of tryptase, histamine, prostaglandin D(2), and peroxidase activity (p<0.05 v basal period) but not eosinophil cationic protein. The increased release of these mediators was associated with a notable water secretory response.

Conclusions: These results suggest that human intestinal hypersensitivity reactions are characterised by prompt activation of mast cells and other immune cells, with notable and immediate secretion of water and inflammatory mediators into the intestinal lumen. Analysis of the profile of markers released into the jejunum after food provocation may be useful for the objective diagnosis of food allergy.

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Figures

Figure 1

Effect of antigen challenge on jejunal release of (A) tryptase, (B) prostaglandin D2 (PGD2), (C) histamine, and (D) water flux. As data for tryptase, histamine, and PGD2 were not normally distributed, these variables are represented as median and 25th/75th percentiles of six to eight subjects. Water flux data followed a normal distribution and thus each point is represented as mean (SD) from six to eight subjects. Statistical analysis was performed with non-parametric or parametric tests according to the distribution of data. *p<0.05, ***p<0.001 v time matched controls.

Figure 2

Effect of antigen challenge on jejunal release of (A) peroxidase activity and (B) eosinophilic cationic protein (ECP). Each point represents median and 25th/75th percentiles for six to eight subjects. *p<0.05 v time matched control.

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Characterisation of immune mediator release during the immediate response to segmental mucosal challenge in the jejunum of patients with food allergy - PubMed (original) (raw)