Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid - PubMed (original) (raw)

. 1999 Sep 9;401(6749):133-41.

doi: 10.1038/43613.

Affiliations

• PMID: 10490020
• DOI: 10.1038/43613

Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid

A Schmidt et al. Nature. 1999.

Abstract

Endophilin I is a presynaptic protein of unknown function that binds to dynamin, a GTPase that is implicated in endocytosis and recycling of synaptic vesicles. Here we show that endophilin I is essential for the formation of synaptic-like microvesicles (SLMVs) from the plasma membrane. Endophilin I exhibits lysophosphatidic acid acyl transferase (LPAAT) activity, and endophilin-I-mediated SLMV formation requires the transfer of the unsaturated fatty acid arachidonate to lysophosphatidic acid, converting it to phosphatidic acid. A deletion mutant lacking the SH3 domain through which endophilin I interacts with dynamin still exhibits LPAAT activity but no longer mediates SLMV formation. These results indicate that endophilin I may induce negative membrane curvature by converting an inverted-cone-shaped lipid to a cone-shaped lipid in the cytoplasmic leaflet of the bilayer. We propose that, through this action, endophilin I works with dynamin to mediate synaptic vesicle invagination from the plasma membrane and fission.

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Comment in

• Lipid membranes shape up.
  Scales SJ, Scheller RH. Scales SJ, et al. Nature. 1999 Sep 9;401(6749):123-4. doi: 10.1038/43582. Nature. 1999. PMID: 10490016 No abstract available.

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