Induction of apoptosis in Herpesvirus saimiri-immortalized T lymphocytes by blocking interaction of CD28 with CD80/CD86 - PubMed (original) (raw)
. 1999 Sep 24;263(2):352-6.
doi: 10.1006/bbrc.1999.1364.
Affiliations
- PMID: 10491296
- DOI: 10.1006/bbrc.1999.1364
Induction of apoptosis in Herpesvirus saimiri-immortalized T lymphocytes by blocking interaction of CD28 with CD80/CD86
H Akari et al. Biochem Biophys Res Commun. 1999.
Abstract
We have previously shown that Herpesvirus saimiri (HVS) immortalizes primary macaque monkey T lymphocytes. In this study, we examined the characteristics of the immortalized T cells. The cells showed the phenotype of activated T lymphoblasts (CD3(+) CD25(+) CD69(+) MHC-IIDR(+)) and produced no infectious virus while viral DNA was detected in the Hirt DNA. Interestingly, both a major costimulatory molecule, CD28, and its ligands, CD80/CD86, were coexpressed on the immortalized T cells. The treatment of the cells with a neutralizing monoclonal antibody against CD28, which blocks interaction of CD28 with CD80/CD86, resulted in retarded cell growth and in induction of apoptosis. The effect of the antibody treatment was not overcome by exogenous interleukin-2 treatment. These findings demonstrate the requirement of interaction of CD28 with CD80/CD86 for the optimal growth of HVS-immortalized T cells.
Copyright 1999 Academic Press.
Similar articles
- Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.
Giguère JF, Bounou S, Paquette JS, Madrenas J, Tremblay MJ. Giguère JF, et al. J Virol. 2004 Jun;78(12):6222-32. doi: 10.1128/JVI.78.12.6222-6232.2004. J Virol. 2004. PMID: 15163715 Free PMC article. - Time courses of B7 family molecules expressed on activated T-cells and their biological significance.
Sun ZW, Qiu YH, Shi YJ, Tao R, Chen J, Ge Y, Hu YM, Ma HB, Shi Q, Zhang XG. Sun ZW, et al. Cell Immunol. 2005 Jul-Aug;236(1-2):146-53. doi: 10.1016/j.cellimm.2005.08.021. Epub 2005 Sep 12. Cell Immunol. 2005. PMID: 16165113 - A novel bispecific tetravalent antibody fusion protein to target costimulatory activity for T-cell activation to tumor cells overexpressing ErbB2/HER2.
Biburger M, Weth R, Wels WS. Biburger M, et al. J Mol Biol. 2005 Mar 11;346(5):1299-311. doi: 10.1016/j.jmb.2004.12.052. Epub 2005 Jan 22. J Mol Biol. 2005. PMID: 15713482 - Mechanisms of cell transformation by Herpesvirus saimiri.
Tsygankov AY, Romano G. Tsygankov AY, et al. Anticancer Res. 1999 Mar-Apr;19(2A):973-83. Anticancer Res. 1999. PMID: 10368641 Review. - Herpesvirus saimiri as a model for gammaherpesvirus oncogenesis.
Jung JU, Choi JK, Ensser A, Biesinger B. Jung JU, et al. Semin Cancer Biol. 1999 Jun;9(3):231-9. doi: 10.1006/scbi.1998.0115. Semin Cancer Biol. 1999. PMID: 10343074 Review.
Cited by
- Species-Specific Valid Ternary Interactions of HIV-1 Env-gp120, CD4, and CCR5 as Revealed by an Adaptive Single-Amino Acid Substitution at the V3 Loop Tip.
Koma T, Yokoyama M, Kotani O, Doi N, Nakanishi N, Okubo H, Adachi S, Adachi A, Sato H, Nomaguchi M. Koma T, et al. J Virol. 2021 Jun 10;95(13):e0217720. doi: 10.1128/JVI.02177-20. Epub 2021 Jun 10. J Virol. 2021. PMID: 33883222 Free PMC article. - Commentary: Derivation of Simian Tropic HIV-1 Infectious Clone Reveals Virus Adaptation to a New Host.
Adachi A, Koma T, Doi N, Nomaguchi M. Adachi A, et al. Front Cell Infect Microbiol. 2020 May 15;10:235. doi: 10.3389/fcimb.2020.00235. eCollection 2020. Front Cell Infect Microbiol. 2020. PMID: 32500043 Free PMC article. No abstract available. - Concomitant Enhancement of HIV-1 Replication Potential and Neutralization-Resistance in Concert With Three Adaptive Mutations in Env V1/C2/C4 Domains.
Doi N, Yokoyama M, Koma T, Kotani O, Sato H, Adachi A, Nomaguchi M. Doi N, et al. Front Microbiol. 2019 Jan 17;10:2. doi: 10.3389/fmicb.2019.00002. eCollection 2019. Front Microbiol. 2019. PMID: 30705669 Free PMC article. - CXCR4- and CCR5-Tropic HIV-1 Clones Are Both Tractable to Grow in Rhesus Macaques.
Doi N, Miura T, Mori H, Sakawaki H, Koma T, Adachi A, Nomaguchi M. Doi N, et al. Front Microbiol. 2018 Oct 18;9:2510. doi: 10.3389/fmicb.2018.02510. eCollection 2018. Front Microbiol. 2018. PMID: 30405570 Free PMC article. - In silico Analysis of HIV-1 Env-gp120 Reveals Structural Bases for Viral Adaptation in Growth-Restrictive Cells.
Yokoyama M, Nomaguchi M, Doi N, Kanda T, Adachi A, Sato H. Yokoyama M, et al. Front Microbiol. 2016 Feb 9;7:110. doi: 10.3389/fmicb.2016.00110. eCollection 2016. Front Microbiol. 2016. PMID: 26903989 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials