Preoperative diagnosis of thyroid papillary and anaplastic carcinomas by real-time quantitative reverse transcription-polymerase chain reaction of oncofetal fibronectin messenger RNA - PubMed (original) (raw)

. 1999 Sep 15;59(18):4542-5.

Affiliations

PMID: 10493503

Preoperative diagnosis of thyroid papillary and anaplastic carcinomas by real-time quantitative reverse transcription-polymerase chain reaction of oncofetal fibronectin messenger RNA

T Takano et al. Cancer Res. 1999.

Abstract

The restricted expression of oncofetal fibronectin (onfFN) mRNA in thyroid papillary and anaplastic carcinomas was recently reported. In this study, we measured the copy number of onfFN mRNA in RNAs extracted from fine needle aspiration biopsies by real-time quantitative reverse transcription-PCR using thyroglobulin mRNA as an internal control. By measuring the onfFN:thyroglobulin mRNA ratio, preoperative aspirates from 31 papillary carcinomas and an anaplastic carcinoma can be distinguished from those from 5 adenomatous goiters, 5 follicular adenomas, and 4 follicular carcinomas. Thus, quantification of onfFN by real-time quantitative reverse transcription-PCR may be useful for the preoperative diagnosis of papillary and anaplastic carcinomas.

PubMed Disclaimer

Cited by

High-throughput differential screening of mRNAs by serial analysis of gene expression: decreased expression of trefoil factor 3 mRNA in thyroid follicular carcinomas.
Takano T, Miyauchi A, Yoshida H, Kuma K, Amino N. Takano T, et al. Br J Cancer. 2004 Apr 19;90(8):1600-5. doi: 10.1038/sj.bjc.6601702. Br J Cancer. 2004. PMID: 15083192 Free PMC article.
Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines.
Yamada H, Takano T, Matsuzuka F, Watanabe M, Miyauchi A, Iwatani Y. Yamada H, et al. Genet Mol Biol. 2011 Jan;34(1):6-10. doi: 10.1590/S1415-47572010005000109. Epub 2011 Mar 1. Genet Mol Biol. 2011. PMID: 21637535 Free PMC article.
Gene expression in papillary thyroid carcinoma reveals highly consistent profiles.
Huang Y, Prasad M, Lemon WJ, Hampel H, Wright FA, Kornacker K, LiVolsi V, Frankel W, Kloos RT, Eng C, Pellegata NS, de la Chapelle A. Huang Y, et al. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15044-9. doi: 10.1073/pnas.251547398. Proc Natl Acad Sci U S A. 2001. PMID: 11752453 Free PMC article.
Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy.
Takano T, Miyauchi A, Yoshida H, Hasegawa Y, Kuma K, Amino N. Takano T, et al. Br J Cancer. 2001 Jul 6;85(1):102-6. doi: 10.1054/bjoc.2001.1904. Br J Cancer. 2001. PMID: 11437410 Free PMC article.
Gene expression profiles in thyroid carcinomas.
Takano T, Hasegawa Y, Matsuzuka F, Miyauchi A, Yoshida H, Higashiyama T, Kuma K, Amino N. Takano T, et al. Br J Cancer. 2000 Dec;83(11):1495-502. doi: 10.1054/bjoc.2000.1483. Br J Cancer. 2000. PMID: 11076659 Free PMC article.

Preoperative diagnosis of thyroid papillary and anaplastic carcinomas by real-time quantitative reverse transcription-polymerase chain reaction of oncofetal fibronectin messenger RNA - PubMed (original) (raw)