A generic protein purification method for protein complex characterization and proteome exploration - PubMed (original) (raw)
. 1999 Oct;17(10):1030-2.
doi: 10.1038/13732.
Affiliations
- PMID: 10504710
- DOI: 10.1038/13732
A generic protein purification method for protein complex characterization and proteome exploration
G Rigaut et al. Nat Biotechnol. 1999 Oct.
Abstract
We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.
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