Est1 and Cdc13 as comediators of telomerase access - PubMed (original) (raw)
Est1 and Cdc13 as comediators of telomerase access
S K Evans et al. Science. 1999.
Abstract
Cdc13 and Est1 are single-strand telomeric DNA binding proteins that contribute to telomere replication in the yeast Saccharomyces cerevisiae. Here it is shown that fusion of Cdc13 to the telomerase-associated Est1 protein results in greatly elongated telomeres. Fusion proteins consisting of mutant versions of Cdc13 or Est1 confer similar telomere elongation, indicating that close physical proximity can bypass telomerase-defective mutations in either protein. Fusing Cdc13 directly to the catalytic core of telomerase allows stable telomere maintenance in the absence of Est1, consistent with a role for Est1 in mediating telomerase access. Telomere length homeostasis therefore is maintained in part by restricting access of telomerase to chromosome termini, but this limiting situation can be overcome by directly tethering telomerase to the telomere.
Similar articles
- New function of CDC13 in positive telomere length regulation.
Meier B, Driller L, Jaklin S, Feldmann HM. Meier B, et al. Mol Cell Biol. 2001 Jul;21(13):4233-45. doi: 10.1128/MCB.21.13.4233-4245.2001. Mol Cell Biol. 2001. PMID: 11390652 Free PMC article. - Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitment.
Grandin N, Damon C, Charbonneau M. Grandin N, et al. Mol Cell Biol. 2000 Nov;20(22):8397-408. doi: 10.1128/MCB.20.22.8397-8408.2000. Mol Cell Biol. 2000. PMID: 11046137 Free PMC article. - Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance.
Nugent CI, Hughes TR, Lue NF, Lundblad V. Nugent CI, et al. Science. 1996 Oct 11;274(5285):249-52. doi: 10.1126/science.274.5285.249. Science. 1996. PMID: 8824190 - Regulation of telomerase by telomeric proteins.
Smogorzewska A, de Lange T. Smogorzewska A, et al. Annu Rev Biochem. 2004;73:177-208. doi: 10.1146/annurev.biochem.73.071403.160049. Annu Rev Biochem. 2004. PMID: 15189140 Review. - Positive and negative regulation of telomerase access to the telomere.
Evans SK, Lundblad V. Evans SK, et al. J Cell Sci. 2000 Oct;113 Pt 19:3357-64. doi: 10.1242/jcs.113.19.3357. J Cell Sci. 2000. PMID: 10984427 Review.
Cited by
- Control of telomere length in yeast by SUMOylated PCNA and the Elg1 PCNA unloader.
Singh P, Gazy I, Kupiec M. Singh P, et al. Elife. 2023 Aug 2;12:RP86990. doi: 10.7554/eLife.86990. Elife. 2023. PMID: 37530521 Free PMC article. - RPA-like single-stranded DNA-binding protein complexes including CST serve as specialized processivity factors for polymerases.
Barbour AT, Wuttke DS. Barbour AT, et al. Curr Opin Struct Biol. 2023 Aug;81:102611. doi: 10.1016/j.sbi.2023.102611. Epub 2023 May 26. Curr Opin Struct Biol. 2023. PMID: 37245465 Free PMC article. Review. - A comprehensive map of hotspots of de novo telomere addition in Saccharomyces cerevisiae.
Ngo K, Gittens TH, Gonzalez DI, Hatmaker EA, Plotkin S, Engle M, Friedman GA, Goldin M, Hoerr RE, Eichman BF, Rokas A, Benton ML, Friedman KL. Ngo K, et al. Genetics. 2023 May 26;224(2):iyad076. doi: 10.1093/genetics/iyad076. Genetics. 2023. PMID: 37119805 Free PMC article. - A comprehensive map of hotspots of de novo telomere addition in Saccharomyces cerevisiae.
Ngo K, Gittens TH, Gonzalez DI, Hatmaker EA, Plotkin S, Engle M, Friedman GA, Goldin M, Hoerr RE, Eichman BF, Rokas A, Benton ML, Friedman KL. Ngo K, et al. bioRxiv [Preprint]. 2023 Mar 23:2023.03.20.533556. doi: 10.1101/2023.03.20.533556. bioRxiv. 2023. PMID: 36993206 Free PMC article. Updated. Preprint. - Post-Transcriptional and Post-Translational Modifications in Telomerase Biogenesis and Recruitment to Telomeres.
Shepelev N, Dontsova O, Rubtsova M. Shepelev N, et al. Int J Mol Sci. 2023 Mar 6;24(5):5027. doi: 10.3390/ijms24055027. Int J Mol Sci. 2023. PMID: 36902458 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases