beta-TrCP mediates the signal-induced ubiquitination of IkappaBbeta - PubMed (original) (raw)

. 1999 Oct 15;274(42):29591-4.

doi: 10.1074/jbc.274.42.29591.

Affiliations

• PMID: 10514424
• DOI: 10.1074/jbc.274.42.29591

Free article

beta-TrCP mediates the signal-induced ubiquitination of IkappaBbeta

C Wu et al. J Biol Chem. 1999.

Free article

Abstract

We have examined the role of beta-TrCP (beta-transducin repeat-containing protein) in the ubiquitination and degradation of IkappaBbeta, one of the two major IkappaB isoforms in mammalian cells. We demonstrate that beta-TrCP interacts specifically with IkappaBbeta, and such interaction is dependent on prior phosphorylation of IkappaBbeta on serines 19 and 23. Interaction with beta-TrCP is also necessary for ubiquitination of IkappaBbeta upon stimulation of cells, and deletion of the F-box in beta-TrCP abolishes its ability to ubiquitinate IkappaBbeta. Therefore, these results indicate that beta-TrCP plays a critical role in the activation of NF-kappaB by assembling the ubiquitin ligase complex for both phosphorylated IkappaBalpha and IkappaBbeta.

PubMed Disclaimer

Similar articles

• Signal-induced ubiquitination of IkappaBalpha by the F-box protein Slimb/beta-TrCP.
  Spencer E, Jiang J, Chen ZJ. Spencer E, et al. Genes Dev. 1999 Feb 1;13(3):284-94. doi: 10.1101/gad.13.3.284. Genes Dev. 1999. PMID: 9990853 Free PMC article.
• The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro.
  Winston JT, Strack P, Beer-Romero P, Chu CY, Elledge SJ, Harper JW. Winston JT, et al. Genes Dev. 1999 Feb 1;13(3):270-83. doi: 10.1101/gad.13.3.270. Genes Dev. 1999. PMID: 9990852 Free PMC article.
• Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha.
  Read MA, Brownell JE, Gladysheva TB, Hottelet M, Parent LA, Coggins MB, Pierce JW, Podust VN, Luo RS, Chau V, Palombella VJ. Read MA, et al. Mol Cell Biol. 2000 Apr;20(7):2326-33. doi: 10.1128/MCB.20.7.2326-2333.2000. Mol Cell Biol. 2000. PMID: 10713156 Free PMC article.
• Regulation of NF-κB by ubiquitination and degradation of the IκBs.
  Kanarek N, Ben-Neriah Y. Kanarek N, et al. Immunol Rev. 2012 Mar;246(1):77-94. doi: 10.1111/j.1600-065X.2012.01098.x. Immunol Rev. 2012. PMID: 22435548 Review.
• Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity.
  Karin M, Ben-Neriah Y. Karin M, et al. Annu Rev Immunol. 2000;18:621-63. doi: 10.1146/annurev.immunol.18.1.621. Annu Rev Immunol. 2000. PMID: 10837071 Review.

Cited by

• F-Box Proteins and Cancer.
  Yumimoto K, Yamauchi Y, Nakayama KI. Yumimoto K, et al. Cancers (Basel). 2020 May 15;12(5):1249. doi: 10.3390/cancers12051249. Cancers (Basel). 2020. PMID: 32429232 Free PMC article. Review.
• WDR79 promotes the proliferation of non-small cell lung cancer cells via USP7-mediated regulation of the Mdm2-p53 pathway.
  Sun Y, Cao L, Sheng X, Chen J, Zhou Y, Yang C, Deng T, Ma H, Feng P, Liu J, Tan W, Ye M. Sun Y, et al. Cell Death Dis. 2017 Apr 13;8(4):e2743. doi: 10.1038/cddis.2017.162. Cell Death Dis. 2017. PMID: 28406480 Free PMC article.
• Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response.
  Yu Z, Chen T, Li X, Yang M, Tang S, Zhu X, Gu Y, Su X, Xia M, Li W, Zhang X, Wang Q, Cao X, Wang J. Yu Z, et al. Elife. 2016 Apr 11;5:e14087. doi: 10.7554/eLife.14087. Elife. 2016. PMID: 27063938 Free PMC article.
• BioID-based Identification of Skp Cullin F-box (SCF)β-TrCP1/2 E3 Ligase Substrates.
  Coyaud E, Mis M, Laurent EM, Dunham WH, Couzens AL, Robitaille M, Gingras AC, Angers S, Raught B. Coyaud E, et al. Mol Cell Proteomics. 2015 Jul;14(7):1781-95. doi: 10.1074/mcp.M114.045658. Epub 2015 Apr 21. Mol Cell Proteomics. 2015. PMID: 25900982 Free PMC article.
• MicroRNA-mediated mechanisms of the cellular stress response in atherosclerosis.
  Schober A, Nazari-Jahantigh M, Weber C. Schober A, et al. Nat Rev Cardiol. 2015 Jun;12(6):361-74. doi: 10.1038/nrcardio.2015.38. Epub 2015 Apr 7. Nat Rev Cardiol. 2015. PMID: 25855604 Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • GlyGen glycoinformatics resource