The nascent polypeptide-associated complex (NAC) of yeast functions in the targeting process of ribosomes to the ER membrane - PubMed (original) (raw)
The nascent polypeptide-associated complex (NAC) of yeast functions in the targeting process of ribosomes to the ER membrane
B Wiedmann et al. FEBS Lett. 1999.
Free article
Abstract
We study here the binding of ribosomes to the endoplasmic reticulum (ER) membrane and its dependence on nascent polypeptide-associated complex (NAC). For this, we use an in vitro translation system in combination with isolated microsomes. Importantly, all components in the system are derived from a single source, Saccharomyces cerevisiae. Ribosome nascent chains (RNCs) of the two naturally occurring invertase species (secreted or cytosolic) were prepared in wild-type, delta alpha NAC or delta alpha beta 1 beta 3 NAC translation lysates and tested for binding to the corresponding microsomal membranes. We provide evidence that NAC prevents binding of RNCs without a signal sequence to yeast membranes. In the absence of NAC, signal-less RNCs are able to bind to ER membranes. However, following puromycin treatment, only very few nascent chains translocate into the lumen, as detected by glycosylation.
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