Regulation of bad phosphorylation and association with Bcl-x(L) by the MAPK/Erk kinase - PubMed (original) (raw)

. 1999 Oct 22;274(43):31108-13.

doi: 10.1074/jbc.274.43.31108.

Affiliations

• PMID: 10521512
• DOI: 10.1074/jbc.274.43.31108

Free article

Regulation of bad phosphorylation and association with Bcl-x(L) by the MAPK/Erk kinase

M P Scheid et al. J Biol Chem. 1999.

Free article

Abstract

Phosphorylation of the Bcl-2 family protein Bad may represent an important bridge between survival signaling by growth factor receptors and the prevention of apoptosis. Bad phosphorylation was examined following cytokine stimulation, which revealed phosphorylation on a critical residue, serine 112, in a MEK-dependent manner. Furthermore, Bad phosphorylation also increased on several sites distinct from serine 112 but could not be detected on serine 136, previously thought to be a protein kinase B/Akt-targeted residue. Serine 112 phosphorylation was shown to be absolutely required for dissociation of Bad from Bcl-x(L). These results demonstrate for the first time in mammalian cells the involvement of the Ras-MAPK pathway in the phosphorylation of Bad and the regulation of its function.

PubMed Disclaimer

Similar articles

• Activation of JNK1, RSK2, and MSK1 is involved in serine 112 phosphorylation of Bad by ultraviolet B radiation.
  She QB, Ma WY, Zhong S, Dong Z. She QB, et al. J Biol Chem. 2002 Jul 5;277(27):24039-48. doi: 10.1074/jbc.M109907200. Epub 2002 Apr 30. J Biol Chem. 2002. PMID: 11983683
• Differential sensitivity to apoptosis between the human small and large intestinal mucosae: linkage with segment-specific regulation of BCL-2 homologs and involvement of signaling pathways.
  Gauthier R, Laprise P, Cardin E, Harnois C, Plourde A, Reed JC, Vézina A, Vachon PH. Gauthier R, et al. J Cell Biochem. 2001;82(2):339-55. doi: 10.1002/jcb.1172. J Cell Biochem. 2001. PMID: 11527158
• Sublytic C5b-9-stimulated Schwann cell survival through PI 3-kinase-mediated phosphorylation of BAD.
  Hila S, Soane L, Koski CL. Hila S, et al. Glia. 2001 Oct;36(1):58-67. doi: 10.1002/glia.1095. Glia. 2001. PMID: 11571784
• Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt.
  del Peso L, González-García M, Page C, Herrera R, Nuñez G. del Peso L, et al. Science. 1997 Oct 24;278(5338):687-9. doi: 10.1126/science.278.5338.687. Science. 1997. PMID: 9381178
• Dissociation of cytokine-induced phosphorylation of Bad and activation of PKB/akt: involvement of MEK upstream of Bad phosphorylation.
  Scheid MP, Duronio V. Scheid MP, et al. Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7439-44. doi: 10.1073/pnas.95.13.7439. Proc Natl Acad Sci U S A. 1998. PMID: 9636168 Free PMC article.

Cited by

• A benzimidazole derivative exhibiting antitumor activity blocks EGFR and HER2 activity and upregulates DR5 in breast cancer cells.
  Chu B, Liu F, Li L, Ding C, Chen K, Sun Q, Shen Z, Tan Y, Tan C, Jiang Y. Chu B, et al. Cell Death Dis. 2015 Mar 12;6(3):e1686. doi: 10.1038/cddis.2015.25. Cell Death Dis. 2015. PMID: 25766325 Free PMC article.
• Mitochondrial topoisomerase 1 inhibition induces topological DNA damage and T cell dysfunction in patients with chronic viral infection.
  Dang X, Cao D, Zhao J, Schank M, Khanal S, Nguyen LNT, Wu XY, Zhang Y, Zhang J, Jiang Y, Ning S, Wang L, El Gazzar M, Moorman JP, Yao ZQ. Dang X, et al. Front Cell Infect Microbiol. 2022 Nov 3;12:1026293. doi: 10.3389/fcimb.2022.1026293. eCollection 2022. Front Cell Infect Microbiol. 2022. PMID: 36405960 Free PMC article.
• Fisetin Deters Cell Proliferation, Induces Apoptosis, Alleviates Oxidative Stress and Inflammation in Human Cancer Cells, HeLa.
  Afroze N, Pramodh S, Shafarin J, Bajbouj K, Hamad M, Sundaram MK, Haque S, Hussain A. Afroze N, et al. Int J Mol Sci. 2022 Feb 1;23(3):1707. doi: 10.3390/ijms23031707. Int J Mol Sci. 2022. PMID: 35163629 Free PMC article.
• Cholinergic receptor pathways involved in apoptosis, cell proliferation and neuronal differentiation.
  Resende RR, Adhikari A. Resende RR, et al. Cell Commun Signal. 2009 Aug 27;7:20. doi: 10.1186/1478-811X-7-20. Cell Commun Signal. 2009. PMID: 19712465 Free PMC article.
• E5 protein of human papillomavirus type 16 protects human foreskin keratinocytes from UV B-irradiation-induced apoptosis.
  Zhang B, Spandau DF, Roman A. Zhang B, et al. J Virol. 2002 Jan;76(1):220-31. doi: 10.1128/jvi.76.1.220-231.2002. J Virol. 2002. PMID: 11739687 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • GlyGen glycoinformatics resource
  • PhosphoSitePlus

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal