Introduction of chromosome 7 suppresses telomerase with shortening of telomeres in a human mesothelial cell line - PubMed (original) (raw)
. 1999 Nov 1;252(2):376-82.
doi: 10.1006/excr.1999.4619.
Affiliations
- PMID: 10527627
- DOI: 10.1006/excr.1999.4619
Introduction of chromosome 7 suppresses telomerase with shortening of telomeres in a human mesothelial cell line
K Nakabayashi et al. Exp Cell Res. 1999.
Abstract
Introduction of human chromosome 7 by microcell-mediated chromosome transfer induced senescence in a telomerase-positive human mesothelial cell line, MeT5A. In microcell hybrids which underwent senescence, telomerase activity was decreased before entering senescence and telomeric sequences were shortened as cell division proceeded. Concomitantly, expression of the gene encoding telomerase catalytic subunit was abolished, whereas the genes encoding the RNA component of telomerase and its associated protein TEP1 were not affected. In revertants which arose from such microcell hybrids, telomerase activity was restored and the telomeric sequences were elongated. In microcell hybrids which showed no growth arrest, telomerase activity was unaltered. These results suggest that a putative mortality gene on chromosome 7 negatively regulates the telomere maintenance mechanism in MeT5A.
Copyright 1999 Academic Press.
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