Thermodynamics of single mismatches in RNA duplexes - PubMed (original) (raw)

Comparative Study

. 1999 Oct 26;38(43):14214-23.

doi: 10.1021/bi991186l.

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Comparative Study

Thermodynamics of single mismatches in RNA duplexes

R Kierzek et al. Biochemistry. 1999.

Abstract

The thermodynamic properties and structures of single mismatches in short RNA duplexes were studied in optical melting and imino proton NMR experiments. The free energy increments at 37 degrees C measured for non-GU single mismatches range from -2.6 to 1.7 kcal/mol. These increments depend on the identity of the mismatch, adjacent base pairs, and the position in the helix. UU and AA mismatches are more stable close to a helix end, but GG mismatch stability is essentially unaffected by the position in the helix. Approximations are suggested for predicting stabilities of single mismatches in short RNA duplexes.

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