Mitochondria unfold precursor proteins by unraveling them from their N-termini - PubMed (original) (raw)
Mitochondria unfold precursor proteins by unraveling them from their N-termini
S Huang et al. Nat Struct Biol. 1999 Dec.
Abstract
Protein unfolding is a key step in the life cycle of many proteins, including certain proteins that are degraded by ATP-dependent proteases or translocated across membranes. The detailed mechanisms of these unfolding processes are not understood. Precursor proteins are unfolded and imported into mitochondria by a macromolecular machine that spans two membranes and contains at least nine different proteins. Here we examine import of a model precursor protein derived from the ribonuclease barnase and show that mitochondria unfold this protein by unraveling it from its N-terminus. Because barnase in free-solution unfolds by a different pathway, our results demonstrate that mitochondria catalyze unfolding in the way that enzymes catalyze reactions, namely by changing reaction pathways. The effectiveness of this mechanism depends on the structure of the N-terminal part of the precursor protein.
Comment in
- Integrative approaches to protein folding.
[No authors listed] [No authors listed] Nat Struct Biol. 1999 Dec;6(12):1079-80. doi: 10.1038/70001. Nat Struct Biol. 1999. PMID: 10581538 No abstract available. - Protein unfolding: mitochondria offer a helping hand.
Hebert DN. Hebert DN. Nat Struct Biol. 1999 Dec;6(12):1084-5. doi: 10.1038/70006. Nat Struct Biol. 1999. PMID: 10581540
Similar articles
- Active unfolding of precursor proteins during mitochondrial protein import.
Matouschek A, Azem A, Ratliff K, Glick BS, Schmid K, Schatz G. Matouschek A, et al. EMBO J. 1997 Nov 17;16(22):6727-36. doi: 10.1093/emboj/16.22.6727. EMBO J. 1997. PMID: 9362487 Free PMC article. - ATP-dependent proteases degrade their substrates by processively unraveling them from the degradation signal.
Lee C, Schwartz MP, Prakash S, Iwakura M, Matouschek A. Lee C, et al. Mol Cell. 2001 Mar;7(3):627-37. doi: 10.1016/s1097-2765(01)00209-x. Mol Cell. 2001. PMID: 11463387 - Protein unfolding by the mitochondrial membrane potential.
Huang S, Ratliff KS, Matouschek A. Huang S, et al. Nat Struct Biol. 2002 Apr;9(4):301-7. doi: 10.1038/nsb772. Nat Struct Biol. 2002. PMID: 11887183 - Protein unfolding by mitochondria. The Hsp70 import motor.
Matouschek A, Pfanner N, Voos W. Matouschek A, et al. EMBO Rep. 2000 Nov;1(5):404-10. doi: 10.1093/embo-reports/kvd093. EMBO Rep. 2000. PMID: 11258479 Free PMC article. Review. - Protein unfolding--an important process in vivo?
Matouschek A. Matouschek A. Curr Opin Struct Biol. 2003 Feb;13(1):98-109. doi: 10.1016/s0959-440x(03)00010-1. Curr Opin Struct Biol. 2003. PMID: 12581666 Review.
Cited by
- Role of the membrane potential in mitochondrial protein unfolding and import.
Sato TK, Kawano S, Endo T. Sato TK, et al. Sci Rep. 2019 May 21;9(1):7637. doi: 10.1038/s41598-019-44152-z. Sci Rep. 2019. PMID: 31114030 Free PMC article. - Substrate selection by the proteasome through initiation regions.
Tomita T, Matouschek A. Tomita T, et al. Protein Sci. 2019 Jul;28(7):1222-1232. doi: 10.1002/pro.3642. Epub 2019 May 23. Protein Sci. 2019. PMID: 31074920 Free PMC article. Review. - Cotranslocational processing of the protein substrate calmodulin by an AAA+ unfoldase occurs via unfolding and refolding intermediates.
Augustyniak R, Kay LE. Augustyniak R, et al. Proc Natl Acad Sci U S A. 2018 May 22;115(21):E4786-E4795. doi: 10.1073/pnas.1721811115. Epub 2018 May 7. Proc Natl Acad Sci U S A. 2018. PMID: 29735657 Free PMC article. - Tailored Polyproteins Using Sequential Staple and Cut.
Garg S, Singaraju GS, Yengkhom S, Rakshit S. Garg S, et al. Bioconjug Chem. 2018 May 16;29(5):1714-1719. doi: 10.1021/acs.bioconjchem.8b00163. Epub 2018 Apr 30. Bioconjug Chem. 2018. PMID: 29671584 Free PMC article. - Hypothesis: The unfolding power of protein dielectricity.
Uversky VN. Uversky VN. Intrinsically Disord Proteins. 2013 Jul 11;1(1):e25725. doi: 10.4161/idp.25725. eCollection 2013 Jan-Dec. Intrinsically Disord Proteins. 2013. PMID: 28516018 Free PMC article.