Bacterial/CpG DNA down-modulates colony stimulating factor-1 receptor surface expression on murine bone marrow-derived macrophages with concomitant growth arrest and factor-independent survival - PubMed (original) (raw)
. 1999 Dec 15;163(12):6541-50.
Affiliations
- PMID: 10586047
Bacterial/CpG DNA down-modulates colony stimulating factor-1 receptor surface expression on murine bone marrow-derived macrophages with concomitant growth arrest and factor-independent survival
D P Sester et al. J Immunol. 1999.
Abstract
Unmethylated CpG motifs within bacterial DNA constitute a pathogen-associated molecular pattern recognized by the innate immune system. Many of the immunomodulatory functions of bacterial DNA can be ascribed to the ability to activate macrophages and dendritic cells. Here we show stimulatory DNA, like LPS, caused growth arrest of murine bone marrow-derived macrophages proliferating in CSF-1. Stimulatory DNA caused selective down-modulation of CSF-1 receptor surface expression. Flow cytometric analysis of CSF-1-deprived bone marrow-derived macrophages revealed that in contrast to the synchronous reduction of CSF-1 receptor upon CSF-1 addition, activating DNA (both bacterial DNA and CpG-containing oligonucleotide) caused rapid removal of receptor from individual cells leading to a bimodal distribution of surface expression at intermediate times or submaximal doses of stimulus. Despite causing growth arrest, both stimulatory DNA and LPS promoted factor-independent survival of bone marrow-derived macrophages, which was associated with phosphorylation of the mitogen-activated protein kinase family members, extracellular-regulated kinase 1 and 2. CSF-1 receptor down-modulation may polarize the professional APC compartment to the more immunostimulatory dendritic cell-like phenotype by suppressing terminal macrophage differentiation mediated by CSF-1.
Similar articles
- PU.1 and ICSBP control constitutive and IFN-gamma-regulated Tlr9 gene expression in mouse macrophages.
Schroder K, Lichtinger M, Irvine KM, Brion K, Trieu A, Ross IL, Ravasi T, Stacey KJ, Rehli M, Hume DA, Sweet MJ. Schroder K, et al. J Leukoc Biol. 2007 Jun;81(6):1577-90. doi: 10.1189/jlb.0107036. Epub 2007 Mar 14. J Leukoc Biol. 2007. PMID: 17360957 - DNA and its cationic lipid complexes induce CpG motif-dependent activation of murine dendritic cells.
Yoshinaga T, Yasuda K, Ogawa Y, Nishikawa M, Takakura Y. Yoshinaga T, et al. Immunology. 2007 Mar;120(3):295-302. doi: 10.1111/j.1365-2567.2006.02451.x. Epub 2006 Dec 20. Immunology. 2007. PMID: 17199803 Free PMC article. - cAMP inhibits CSF-1-stimulated tyrosine phosphorylation but augments CSF-1R-mediated macrophage differentiation and ERK activation.
Wilson NJ, Cross M, Nguyen T, Hamilton JA. Wilson NJ, et al. FEBS J. 2005 Aug;272(16):4141-52. doi: 10.1111/j.1742-4658.2005.04826.x. FEBS J. 2005. PMID: 16098196 - The actions of bacterial DNA on murine macrophages.
Sester DP, Stacey KJ, Sweet MJ, Beasley SJ, Cronau SL, Hume DA. Sester DP, et al. J Leukoc Biol. 1999 Oct;66(4):542-8. doi: 10.1002/jlb.66.4.542. J Leukoc Biol. 1999. PMID: 10534106 Review. - Regulation of proliferation of bone marrow-derived macrophages.
Hume DA, Allan W, Fabrus B, Weidemann MJ, Hapel AJ, Bartelmez S. Hume DA, et al. Lymphokine Res. 1987 Spring;6(2):127-39. Lymphokine Res. 1987. PMID: 3035291 Review.
Cited by
- CD115- monocytic myeloid-derived suppressor cells are precursors of OLFM4high polymorphonuclear myeloid-derived suppressor cells.
Zou Y, Kamada N, Seong SY, Seo SU. Zou Y, et al. Commun Biol. 2023 Mar 15;6(1):272. doi: 10.1038/s42003-023-04650-3. Commun Biol. 2023. PMID: 36922564 Free PMC article. - CSF1R as a Therapeutic Target in Bone Diseases: Obvious but Not so Simple.
Hume DA, Batoon L, Sehgal A, Keshvari S, Irvine KM. Hume DA, et al. Curr Osteoporos Rep. 2022 Dec;20(6):516-531. doi: 10.1007/s11914-022-00757-4. Epub 2022 Oct 5. Curr Osteoporos Rep. 2022. PMID: 36197652 Free PMC article. Review. - Amino acids suppress macropinocytosis and promote release of CSF1 receptor in macrophages.
Mendel ZI, Reynolds MB, Abuaita BH, O'Riordan MX, Swanson JA. Mendel ZI, et al. J Cell Sci. 2022 Feb 15;135(4):jcs259284. doi: 10.1242/jcs.259284. Epub 2022 Feb 21. J Cell Sci. 2022. PMID: 35107133 Free PMC article. - A transcriptional landscape of 28 porcine tissues obtained by super deepSAGE sequencing.
Huang T, Yang M, Dong K, Xu M, Liu J, Chen Z, Zhu S, Chen W, Yin J, Jin K, Deng Y, Guan Z, Huang X, Yang J, Han R, Yao M. Huang T, et al. BMC Genomics. 2020 Mar 14;21(1):229. doi: 10.1186/s12864-020-6628-7. BMC Genomics. 2020. PMID: 32171242 Free PMC article. - Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system.
Wu Z, Harne R, Chintoan-Uta C, Hu TJ, Wallace R, MacCallum A, Stevens MP, Kaiser P, Balic A, Hume DA. Wu Z, et al. Dev Comp Immunol. 2020 Apr;105:103586. doi: 10.1016/j.dci.2019.103586. Epub 2019 Dec 20. Dev Comp Immunol. 2020. PMID: 31870792 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials
Miscellaneous