Ex vivo generation of CD34(+) cells from CD34(-) hematopoietic cells - PubMed (original) (raw)
. 1999 Dec 15;94(12):4053-9.
Affiliations
- PMID: 10590049
Free article
Ex vivo generation of CD34(+) cells from CD34(-) hematopoietic cells
Y Nakamura et al. Blood. 1999.
Free article
Abstract
The human Lin(-)CD34(-) cell population contains a newly defined class of hematopoietic stem cells that reconstitute hematopoiesis in xenogeneic transplantation systems. We therefore developed a culture condition in which these cells were maintained and then acquired CD34 expression and the ability to produce colony-forming cells (CFC) and SCID-repopulating cells (SRCs). A murine bone marrow stromal cell line, HESS-5, supports the survival and proliferation of Lin(-)CD34(-) cells in the presence of fetal calf serum and human cytokines thrombopoietin, Flk-2/Flt-3 ligand, stem cell factor, granulocyte colony-stimulating factor, interleukin-3, and interleukin-6. Although Lin(-)CD34(-) cells do not initially form any hematopoietic colonies in methylcellulose, they do acquire the colony-forming ability during 7 days of culture, which coincides with their conversion to a CD34(+) phenotype. From 2.2% to 12.1% of the cells became positive for CD34 after culture. The long-term multilineage repopulating ability of these cultured cells was also confirmed by transplantation into irradiated NOD/SCID mice. These results represent the first in vitro demonstration of the precursor of CD34(+) cells in the human CD34(-) cell population. Furthermore, the in vitro system we reported here is expected to open the way to the precise characterization and ex vivo manipulation of Lin(-)CD34(-) hematopoietic stem cells.
Similar articles
- Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system.
Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T. Ando K, et al. Exp Hematol. 2000 Jun;28(6):690-9. doi: 10.1016/s0301-472x(00)00154-5. Exp Hematol. 2000. PMID: 10880755 - Dissociation between stem cell phenotype and NOD/SCID repopulating activity in human peripheral blood CD34(+) cells after ex vivo expansion.
Danet GH, Lee HW, Luongo JL, Simon MC, Bonnet DA. Danet GH, et al. Exp Hematol. 2001 Dec;29(12):1465-73. doi: 10.1016/s0301-472x(01)00750-0. Exp Hematol. 2001. PMID: 11750106 - In vitro and in vivo evidence for the long-term multilineage (myeloid, B, NK, and T) reconstitution capacity of ex vivo expanded human CD34(+) cord blood cells.
Kobari L, Pflumio F, Giarratana M, Li X, Titeux M, Izac B, Leteurtre F, Coulombel L, Douay L. Kobari L, et al. Exp Hematol. 2000 Dec;28(12):1470-80. doi: 10.1016/s0301-472x(00)00557-9. Exp Hematol. 2000. PMID: 11146169 - Functional analysis of human hematopoietic repopulating cells mobilized with granulocyte colony-stimulating factor alone versus granulocyte colony-stimulating factor in combination with stem cell factor.
Hess DA, Levac KD, Karanu FN, Rosu-Myles M, White MJ, Gallacher L, Murdoch B, Keeney M, Ottowski P, Foley R, Chin-Yee I, Bhatia M. Hess DA, et al. Blood. 2002 Aug 1;100(3):869-78. doi: 10.1182/blood.v100.3.869. Blood. 2002. PMID: 12130497 - Differential kinetics of primitive hematopoietic cells assayed in vitro and in vivo during serum-free suspension culture of CD34+ blood progenitor cells.
Möbest D, Goan SR, Junghahn I, Winkler J, Fichtner I, Hermann M, Becker M, de Lima-Hahn E, Henschler R. Möbest D, et al. Stem Cells. 1999;17(3):152-61. doi: 10.1002/stem.170152. Stem Cells. 1999. PMID: 10342558
Cited by
- Changes in the proliferative activity of human hematopoietic stem cells in NOD/SCID mice and enhancement of their transplantability after in vivo treatment with cell cycle inhibitors.
Cashman J, Dykstra B, Clark-Lewis I, Eaves A, Eaves C. Cashman J, et al. J Exp Med. 2002 Nov 4;196(9):1141-9. doi: 10.1084/jem.20010916. J Exp Med. 2002. PMID: 12417625 Free PMC article. - Biology of normal and acute myeloid leukemia stem cells.
Dick JE, Lapidot T. Dick JE, et al. Int J Hematol. 2005 Dec;82(5):389-96. doi: 10.1532/IJH97.05144. Int J Hematol. 2005. PMID: 16533740 Review. - Previously undetected human hematopoietic cell populations with short-term repopulating activity selectively engraft NOD/SCID-beta2 microglobulin-null mice.
Glimm H, Eisterer W, Lee K, Cashman J, Holyoake TL, Nicolini F, Shultz LD, von Kalle C, Eaves CJ. Glimm H, et al. J Clin Invest. 2001 Jan;107(2):199-206. doi: 10.1172/JCI11519. J Clin Invest. 2001. PMID: 11160136 Free PMC article. - Extracellular vesicles from human iPSCs enhance reconstitution capacity of cord blood-derived hematopoietic stem and progenitor cells.
Karnas E, Sekuła-Stryjewska M, Kmiotek-Wasylewska K, Bobis-Wozowicz S, Ryszawy D, Sarna M, Madeja Z, Zuba-Surma EK. Karnas E, et al. Leukemia. 2021 Oct;35(10):2964-2977. doi: 10.1038/s41375-021-01325-y. Epub 2021 Jun 17. Leukemia. 2021. PMID: 34140648 Free PMC article. - Intraarterial injection of muscle-derived CD34(+)Sca-1(+) stem cells restores dystrophin in mdx mice.
Torrente Y, Tremblay JP, Pisati F, Belicchi M, Rossi B, Sironi M, Fortunato F, El Fahime M, D'Angelo MG, Caron NJ, Constantin G, Paulin D, Scarlato G, Bresolin N. Torrente Y, et al. J Cell Biol. 2001 Jan 22;152(2):335-48. doi: 10.1083/jcb.152.2.335. J Cell Biol. 2001. PMID: 11266450 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical