Investigation into the acyl chain packing of endotoxins and phospholipids under near physiological conditions by WAXS and FTIR spectroscopy - PubMed (original) (raw)
. 1999 Dec 15;128(2):175-86.
doi: 10.1006/jsbi.1999.4186.
Affiliations
- PMID: 10600571
- DOI: 10.1006/jsbi.1999.4186
Investigation into the acyl chain packing of endotoxins and phospholipids under near physiological conditions by WAXS and FTIR spectroscopy
K Brandenburg et al. J Struct Biol. 1999.
Abstract
The acyl chain packing of various endotoxins and phospholipids was monitored via the main wide-angle reflection between 0.410 and 0.460 nm by wide-angle X-ray scattering (WAXS) and via the absorption band of the symmetric stretching vibration of the methylene groups v(s)(CH(2)) around 2849 to 2853 cm(-1) by Fourier-transform infrared spectroscopy. The lipids investigated included various rough mutant (R) and smooth form (S) lipopolysaccharides (LPS) differing in the length of the sugar portion, lipid A, the "endotoxic principle" of LPS, and various saturated and unsaturated phospholipids with different head groups under a near physiological (>/=85%) water content. The packing density of the saturated endotoxin acyl chains is lower than those of saturated phospholipids but similar to those of monounsaturated phospholipids, each in the gel phase. The hydrophobic moiety of endotoxins thus exhibits significant conformational disorder already in the gel phase. The acyl chain packing of the endotoxins decreases with increasing length of the sugar chain lengths, which seems to be relevant to the observed differences in biological activity. For Re-LPS with different counterions (salt forms), in the presence of externally added cations or at reduced water content (50%), no change of the acyl chain packing density is deduced in the X-ray data, although the FT-IR data indicate its increase. The position of the v(s)(CH(2)) vibration is, thus, only a relative measure of lipid order, in particular when lipids with different head groups and in the presence of external agents are compared.
Copyright 1999 Academic Press.
Similar articles
- Structural polymorphisms of rough mutant lipopolysaccharides Rd to Ra from Salmonella minnesota.
Seydel U, Koch MH, Brandenburg K. Seydel U, et al. J Struct Biol. 1993 May-Jun;110(3):232-43. doi: 10.1006/jsbi.1993.1026. J Struct Biol. 1993. PMID: 8373704 - Biophysical characterization of lipopolysaccharide and lipid A inactivation by lactoferrin.
Brandenburg K, Jürgens G, Müller M, Fukuoka S, Koch MH. Brandenburg K, et al. Biol Chem. 2001 Aug;382(8):1215-25. doi: 10.1515/BC.2001.152. Biol Chem. 2001. PMID: 11592403 - Structural investigations into the interaction of hemoglobin and part structures with bacterial endotoxins.
Howe J, Garidel P, Roessle M, Richter W, Alexander C, Fournier K, Mach JP, Waelli T, Gorczynski RM, Ulmer AJ, Zähringer U, Hartmann A, Rietschel ET, Brandenburg K. Howe J, et al. Innate Immun. 2008 Feb;14(1):39-49. doi: 10.1177/1753425907087257. Innate Immun. 2008. PMID: 18387918 - [New structures recently found in and around the center of biological activities of LPS (endotoxin)].
Kawahara K, Isshiki Y. Kawahara K, et al. Nihon Saikingaku Zasshi. 1995;50(2):451-69. doi: 10.3412/jsb.50.451. Nihon Saikingaku Zasshi. 1995. PMID: 7623413 Review. Japanese. No abstract available. - What we know and don't know about the chemical and physical structure of lipopolysaccharide in relation to biological activity.
Müller-Loennies S, Zähringer U, Seydel U, Kusumoto S, Ulmer AJ, Rietschel ET. Müller-Loennies S, et al. Prog Clin Biol Res. 1998;397:51-72. Prog Clin Biol Res. 1998. PMID: 9575547 Review. No abstract available.
Cited by
- Out of Sight, Out of Mind: The Effect of the Equilibration Protocol on the Structural Ensembles of Charged Glycolipid Bilayers.
Messias A, Santos DES, Pontes FJS, Lima FS, Soares TA. Messias A, et al. Molecules. 2020 Nov 4;25(21):5120. doi: 10.3390/molecules25215120. Molecules. 2020. PMID: 33158044 Free PMC article. - Amphiphilic Aminoglycosides as Medicinal Agents.
Dezanet C, Kempf J, Mingeot-Leclercq MP, Décout JL. Dezanet C, et al. Int J Mol Sci. 2020 Oct 8;21(19):7411. doi: 10.3390/ijms21197411. Int J Mol Sci. 2020. PMID: 33049963 Free PMC article. Review. - The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia.
Yeh LC, Chen SP, Liao FH, Wu TH, Huang YT, Lin SY. Yeh LC, et al. Int J Mol Sci. 2020 Mar 2;21(5):1699. doi: 10.3390/ijms21051699. Int J Mol Sci. 2020. PMID: 32131445 Free PMC article. - Identification of Streptomyces strains isolated from Humulus lupulus rhizosphere and determination of plant growth promotion potential of selected strains.
Özdemir Koçak F. Özdemir Koçak F. Turk J Biol. 2019 Dec 13;43(6):391-403. doi: 10.3906/biy-1906-37. eCollection 2019. Turk J Biol. 2019. PMID: 31892814 Free PMC article. - Polysaccharide Chain Length of Lipopolysaccharides From Salmonella Minnesota Is a Determinant of Aggregate Stability, Plasma Residence Time and Proinflammatory Propensity in vivo.
Sali W, Patoli D, Pais de Barros JP, Labbé J, Deckert V, Duhéron V, Le Guern N, Blache D, Chaumont D, Lesniewska E, Gasquet B, Paul C, Moreau M, Denat F, Masson D, Lagrost L, Gautier T. Sali W, et al. Front Microbiol. 2019 Aug 2;10:1774. doi: 10.3389/fmicb.2019.01774. eCollection 2019. Front Microbiol. 2019. PMID: 31428071 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources