Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase eta - PubMed (original) (raw)
. 1999 Dec 24;274(52):36835-8.
doi: 10.1074/jbc.274.52.36835.
Affiliations
- PMID: 10601233
- DOI: 10.1074/jbc.274.52.36835
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Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase eta
M T Washington et al. J Biol Chem. 1999.
Free article
Abstract
The yeast RAD30 gene functions in error-free replication of UV-damaged DNA, and RAD30 encodes a DNA polymerase, pol eta, that has the ability to efficiently and correctly replicate past a cis-syn-thymine-thymine dimer in template DNA. To better understand the role of pol eta in damage bypass, we examined its fidelity and processivity on nondamaged DNA templates. Steady-state kinetic analyses of deoxynucleotide incorporation indicate that pol eta has a low fidelity, misincorporating deoxynucleotides with a frequency of about 10(-2) to 10(-3). Also pol eta has a low processivity, incorporating only a few nucleotides before dissociating. We suggest that pol eta's low fidelity reflects a flexibility in its active site rendering it more tolerant of DNA damage, while its low processivity limits its activity to reduce errors.
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