Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles - PubMed (original) (raw)
Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles
C Thiele et al. Nat Cell Biol. 2000 Jan.
Abstract
Here, to study lipid-protein interactions that contribute to the biogenesis of regulated secretory vesicles, we have developed new approaches by which to label proteins in vivo, using photoactivatable cholesterol and glycerophospholipids. We identify synaptophysin as a major specifically cholesterol-binding protein in PC12 cells and brain synaptic vesicles. Limited cholesterol depletion, which has little effect on total endocytic activity, blocks the biogenesis of synaptic-like microvesicles (SLMVs) from the plasma membrane. We propose that specific interactions between cholesterol and SLMV membrane proteins, such as synaptophysin, contribute to both the segregation of SLMV membrane constituents from plasma-membrane constituents, and the induction of synaptic-vesicle curvature.
Comment in
- Racing lipid rafts for synaptic-vesicle formation.
Martin TF. Martin TF. Nat Cell Biol. 2000 Jan;2(1):E9-11. doi: 10.1038/71392. Nat Cell Biol. 2000. PMID: 10620814 No abstract available.
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