Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites - PubMed (original) (raw)
. 2000 Feb;127(3):643-53.
doi: 10.1242/dev.127.3.643.
Affiliations
- PMID: 10631184
- DOI: 10.1242/dev.127.3.643
Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites
A Manaia et al. Development. 2000 Feb.
Abstract
It is now widely accepted that hemopoietic cells born intraembryonically are the best candidates for the seeding of definitive hemopoietic organs. To further understand the mechanisms involved in the generation of definitive hemopoietic stem cells, we analysed the expression of the hemopoietic-related transcription factors Lmo2 and GATA-3 during the early steps of mouse development (7-12 dpc), with a particular emphasis on intraembryonic hemogenic sites. We show here that both Lmo2 and GATA-3 are present in the intraembryonic regions known to give rise to hemopoietic precursors in vitro and in vivo, suggesting that they act together at key points of hemopoietic development. (1) Lmo2 and GATA-3 are expressed in the caudal mesoderm during the phase of intraembryonic precursors determination. (2) A highly transient concomitant expression is observed in the caudal intraembryonic definitive endoderm, suggesting that these factors are involved in the specification of intraembryonic hemopoietic precursors. (3) Lmo2 and GATA-3 are expressed within the hemopoietic clusters located in the aortic floor during fetal liver colonisation. Furthermore, a strong GATA-3 signal allowed us to uncover previously unreported mesodermal aggregates beneath the aorta. A combined in situ and immunocytological analysis strongly suggests that ventral mesodermal GATA-3 patches are involved in the process of intraembryonic stem cell generation.
Similar articles
- Hemangioblast commitment in the avian allantois: cellular and molecular aspects.
Caprioli A, Minko K, Drevon C, Eichmann A, Dieterlen-Lièvre F, Jaffredo T. Caprioli A, et al. Dev Biol. 2001 Oct 1;238(1):64-78. doi: 10.1006/dbio.2001.0362. Dev Biol. 2001. PMID: 11783994 - Fli1, Elf1, and Ets1 regulate the proximal promoter of the LMO2 gene in endothelial cells.
Landry JR, Kinston S, Knezevic K, Donaldson IJ, Green AR, Göttgens B. Landry JR, et al. Blood. 2005 Oct 15;106(8):2680-7. doi: 10.1182/blood-2004-12-4755. Epub 2005 Jun 30. Blood. 2005. PMID: 15994290 - Expression of the leukemia oncogene Lmo2 is controlled by an array of tissue-specific elements dispersed over 100 kb and bound by Tal1/Lmo2, Ets, and Gata factors.
Landry JR, Bonadies N, Kinston S, Knezevic K, Wilson NK, Oram SH, Janes M, Piltz S, Hammett M, Carter J, Hamilton T, Donaldson IJ, Lacaud G, Frampton J, Follows G, Kouskoff V, Göttgens B. Landry JR, et al. Blood. 2009 Jun 4;113(23):5783-92. doi: 10.1182/blood-2008-11-187757. Epub 2009 Jan 26. Blood. 2009. PMID: 19171877 - Hemangioblasts and hemopoietic stem cells during ontogeny.
Dieterlen-Lièvre F, Pardanaud L, Bollerot K, Jaffredo T. Dieterlen-Lièvre F, et al. C R Biol. 2002 Oct;325(10):1013-20. doi: 10.1016/s1631-0691(02)01515-9. C R Biol. 2002. PMID: 12494498 Review. - [Embryonic sources of definitive hemopoietic stem cells].
Domaratskaia EI, Starostin VI, Butorina NN. Domaratskaia EI, et al. Izv Akad Nauk Ser Biol. 2001 Nov-Dec;(6):672-81. Izv Akad Nauk Ser Biol. 2001. PMID: 15926333 Review. Russian.
Cited by
- Hematopoiesis: A Layered Organization Across Chordate Species.
Elsaid R, Soares-da-Silva F, Peixoto M, Amiri D, Mackowski N, Pereira P, Bandeira A, Cumano A. Elsaid R, et al. Front Cell Dev Biol. 2020 Dec 16;8:606642. doi: 10.3389/fcell.2020.606642. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 33392196 Free PMC article. Review. - Yolk sac hematopoiesis: does it contribute to the adult hematopoietic system?
Wittamer V, Bertrand JY. Wittamer V, et al. Cell Mol Life Sci. 2020 Oct;77(20):4081-4091. doi: 10.1007/s00018-020-03527-6. Epub 2020 May 13. Cell Mol Life Sci. 2020. PMID: 32405721 Free PMC article. Review. - Mapping cell migrations and fates in a gastruloid model to the human primitive streak.
Martyn I, Siggia ED, Brivanlou AH. Martyn I, et al. Development. 2019 Sep 12;146(17):dev179564. doi: 10.1242/dev.179564. Development. 2019. PMID: 31427289 Free PMC article. - The multi-faceted role of Gata3 in developmental haematopoiesis.
Zaidan N, Ottersbach K. Zaidan N, et al. Open Biol. 2018 Nov 21;8(11):180152. doi: 10.1098/rsob.180152. Open Biol. 2018. PMID: 30463912 Free PMC article. Review. - Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.
Baron CS, Kester L, Klaus A, Boisset JC, Thambyrajah R, Yvernogeau L, Kouskoff V, Lacaud G, van Oudenaarden A, Robin C. Baron CS, et al. Nat Commun. 2018 Jun 28;9(1):2517. doi: 10.1038/s41467-018-04893-3. Nat Commun. 2018. PMID: 29955049 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases