Genetic polymorphism of IgD-like cell surface immunoglobulin in the mouse - PubMed (original) (raw)
Genetic polymorphism of IgD-like cell surface immunoglobulin in the mouse
J W Goding et al. Proc Natl Acad Sci U S A. 1976 Apr.
Abstract
Lymphocyte surface antigens from spleen cells of several mouse strains were studied by cell surface radioiodination, extraction with detergent incubation with various antisera, and separation of complexes using protein A-containing staphylococci as a solid phase adsorbent. Complexes were then dissociated and analyzed by polyacrylamide gel electrophoresis in the presence of sodium diodecyl sulfate. Using this technique and an alloantiserum prepared in C57BL mice against CBA spleen cells, four distinct specific peaks of radioactivity were found with CBA spleen cells. These corresponded to H-2 and Ia antigens, immunoglobulin light chain, and a heavy chain previously proposed to be the murine homolog of the human delta chain. With the same serum, B10.BR spleen cells revealed only H-2 and Ia antigens, whereas C57BL.Ige (allotype congenic) spleen cells showed only the light chain and "delta" chain peaks. Depletion of immunoglobulin from the surface-iodinated preparations resulted in removal of the light chain and "delta" chain peaks. The tissue distribution and membrane expression of this "delta" chain antigen was then studied by indirect immunofluorescence with various C57BL derived alloantisera and lymphoid cells from C57.Ige allotype congenic mice. Significant numbers of positive cells were found in spleen, lymph nodes, and Peyer's patches, whereas few if any positive were found in bone marrow or thymus. No reaction was found between this molecule and alloantisera to any of the previously described immunoglobulin allotypes. It is proposed that these alloantisera to spleen cells recognise one allelic form of the murine "delta" chain coded for by a gene locus closely linked to the known structural genes for mouse immunoglobulin heavy chains. The designation Ig-5 is proposed for this new immunoglobulin heavy chain locus.
Similar articles
- Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D.
Abney ER, Hunter IR, Parkhouse RM. Abney ER, et al. Nature. 1976 Feb 5;259(5542):404-6. doi: 10.1038/259404a0. Nature. 1976. PMID: 1082551 No abstract available. - Antigen-induced co-capping of IgM and IgD-like receptors on murine B cells.
Goding JW, Layton JE. Goding JW, et al. J Exp Med. 1976 Sep 1;144(3):852-7. doi: 10.1084/jem.144.3.852. J Exp Med. 1976. PMID: 822118 Free PMC article. - Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation.
Goding JW. Goding JW. Contemp Top Immunobiol. 1978;8:203-43. doi: 10.1007/978-1-4684-0922-2_7. Contemp Top Immunobiol. 1978. PMID: 357078 Review. - IgD receptors of lymphoid cells.
Pernis B, Forni L. Pernis B, et al. Immunol Commun. 1976;5(9):807-26. doi: 10.3109/08820137609047621. Immunol Commun. 1976. PMID: 799618 Review.
Cited by
- Immunoglobulin isoantigens (allotypes) in the mouse : V. Characterization of IgM allotypes.
Black SJ, Goding JW, Gutman GA, Herzenberg LA, Loken MR, Osborne BA, van der Loo W, Warner NL. Black SJ, et al. Immunogenetics. 1978 Dec;7(1):213-30. doi: 10.1007/BF01844009. Immunogenetics. 1978. PMID: 21302076 - Surface receptors on lymphoreticular cells: sensory devices for host recognition of foreign antigens and neoplasia.
Elliott BE, Kerbel RS, Nagy ZA. Elliott BE, et al. Can Med Assoc J. 1980 Jun 21;122(12):1361-72. Can Med Assoc J. 1980. PMID: 7020898 Free PMC article. Review. - Genetic control of murine IgD structural heterogeneity.
Pollock RR, Dorf ME, Mescher MF. Pollock RR, et al. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4256-9. doi: 10.1073/pnas.77.7.4256. Proc Natl Acad Sci U S A. 1980. PMID: 6933475 Free PMC article. - In vitro tolerance induction of primed, IgD-negative murine spleen cells.
Walker SM, Weigle WO. Walker SM, et al. J Exp Med. 1981 Mar 1;153(3):653-64. doi: 10.1084/jem.153.3.653. J Exp Med. 1981. PMID: 6166703 Free PMC article.
References
- Biochem Biophys Res Commun. 1975 Jul 22;65(2):483-9 - PubMed
- Cell. 1975 Oct;6(2):149-59 - PubMed
- Nature. 1974 Dec 13;252(5484):600-2 - PubMed
- Proc Natl Acad Sci U S A. 1973 Oct;70(10):2879-83 - PubMed
- J Immunol. 1975 Dec;115(6):1617-24 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources