Turning of nerve growth cones induced by localized increases in intracellular calcium ions - PubMed (original) (raw)
. 2000 Jan 6;403(6765):89-93.
doi: 10.1038/47501.
Affiliations
- PMID: 10638759
- DOI: 10.1038/47501
Turning of nerve growth cones induced by localized increases in intracellular calcium ions
J Q Zheng. Nature. 2000.
Abstract
Guidance of developing axons involves turning of the motile tip, the growth cone, in response to a variety of extracellular cues. Little is known about the intracellular mechanism by which the directional signal is transduced. Ca2+ is a key second messenger in growth cone extension and has been implicated in growth-cone turning. Here I report that a direct, spatially restricted elevation of intracellular Ca2+ concentration ([Ca2+]i) on one side of the growth cone by focal laser-induced photolysis (FLIP) of caged Ca2+ consistently induced turning of the growth cone to the side with elevated [Ca2+]i (attraction). Furthermore, when the resting [Ca2+]i at the growth cone was decreased by the removal of extracellular Ca2+, the same focal elevation of [Ca2+]i by FLIP induced repulsion. These results provide direct evidence that a localized Ca2+ signal in the growth cone can provide the intracellular directional cue for extension and is sufficient to initiate both attraction and repulsion. By integrating local and global Ca2+ signals, a growth cone could thus generate different turning responses under different environmental conditions during guidance.
Similar articles
- Calcium signalling in the guidance of nerve growth by netrin-1.
Hong K, Nishiyama M, Henley J, Tessier-Lavigne M, Poo M. Hong K, et al. Nature. 2000 Jan 6;403(6765):93-8. doi: 10.1038/47507. Nature. 2000. PMID: 10638760 - Calcium mediates bidirectional growth cone turning induced by myelin-associated glycoprotein.
Henley JR, Huang KH, Wang D, Poo MM. Henley JR, et al. Neuron. 2004 Dec 16;44(6):909-16. doi: 10.1016/j.neuron.2004.11.030. Neuron. 2004. PMID: 15603734 Free PMC article. - In vivo regulation of axon extension and pathfinding by growth-cone calcium transients.
Gomez TM, Spitzer NC. Gomez TM, et al. Nature. 1999 Jan 28;397(6717):350-5. doi: 10.1038/16927. Nature. 1999. PMID: 9950427 - Attraction vs. repulsion: the growth cone decides.
McFarlane S. McFarlane S. Biochem Cell Biol. 2000;78(5):563-8. Biochem Cell Biol. 2000. PMID: 11103946 Review. - Calcium signalling in growth cone migration.
Bolsover SR. Bolsover SR. Cell Calcium. 2005 May;37(5):395-402. doi: 10.1016/j.ceca.2005.01.007. Cell Calcium. 2005. PMID: 15820386 Review.
Cited by
- Local translation and directional steering in axons.
Lin AC, Holt CE. Lin AC, et al. EMBO J. 2007 Aug 22;26(16):3729-36. doi: 10.1038/sj.emboj.7601808. Epub 2007 Jul 26. EMBO J. 2007. PMID: 17660744 Free PMC article. Review. - The oligodendrocyte growth cone and its actin cytoskeleton: A fundamental element for progenitor cell migration and CNS myelination.
Thomason EJ, Escalante M, Osterhout DJ, Fuss B. Thomason EJ, et al. Glia. 2020 Jul;68(7):1329-1346. doi: 10.1002/glia.23735. Epub 2019 Nov 7. Glia. 2020. PMID: 31696982 Free PMC article. Review. - Bayesian model predicts the response of axons to molecular gradients.
Mortimer D, Feldner J, Vaughan T, Vetter I, Pujic Z, Rosoff WJ, Burrage K, Dayan P, Richards LJ, Goodhill GJ. Mortimer D, et al. Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10296-301. doi: 10.1073/pnas.0900715106. Epub 2009 Jun 18. Proc Natl Acad Sci U S A. 2009. PMID: 19541606 Free PMC article. - Hyperpolarization-activated cyclic nucleotide-gated channels in olfactory sensory neurons regulate axon extension and glomerular formation.
Mobley AS, Miller AM, Araneda RC, Maurer LR, Müller F, Greer CA. Mobley AS, et al. J Neurosci. 2010 Dec 8;30(49):16498-508. doi: 10.1523/JNEUROSCI.4225-10.2010. J Neurosci. 2010. PMID: 21147989 Free PMC article. - Focal loss of actin bundles causes microtubule redistribution and growth cone turning.
Zhou FQ, Waterman-Storer CM, Cohan CS. Zhou FQ, et al. J Cell Biol. 2002 May 27;157(5):839-49. doi: 10.1083/jcb.200112014. Epub 2002 May 28. J Cell Biol. 2002. PMID: 12034775 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous