The development of the action potential mechanism of amphibian neurons isolated in culture - PubMed (original) (raw)

The development of the action potential mechanism of amphibian neurons isolated in culture

N C Spitzer et al. Proc Natl Acad Sci U S A. 1976 May.

Abstract

Nerve and muscle cells differentiated morphologically, in cultures of dissociated cells prepared from amphibian neural plate and underlying mesoderm (Xenopus laevis, Nieuwkoop and Faber stage 15). Cultures were grown in a defined medium containing sterile Steinberg's salt solution and 0.1% bovine serum albumin, and maintained for periods up to 5 days.

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