The Ki-67 protein: from the known and the unknown - PubMed (original) (raw)
Review
The Ki-67 protein: from the known and the unknown
T Scholzen et al. J Cell Physiol. 2000 Mar.
Abstract
The expression of the human Ki-67 protein is strictly associated with cell proliferation. During interphase, the antigen can be exclusively detected within the nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. The fact that the Ki-67 protein is present during all active phases of the cell cycle (G(1), S, G(2), and mitosis), but is absent from resting cells (G(0)), makes it an excellent marker for determining the so-called growth fraction of a given cell population. In the first part of this study, the term proliferation marker is discussed and examples of the applications of anti-Ki-67 protein antibodies in diagnostics of human tumors are given. The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of the disease. The best-studied examples in this context are carcinomas of the prostate and the breast. For these types of tumors, the prognostic value for survival and tumor recurrence has repeatedly been proven in uni- and multivariate analysis. The preparation of new monoclonal antibodies that react with the Ki-67 equivalent protein from rodents now extends the use of the Ki-67 protein as a proliferation marker to laboratory animals that are routinely used in basic research. The second part of this review focuses on the biology of the Ki-67 protein. Our current knowledge of the Ki-67 gene and protein structure, mRNA splicing, expression, and cellular localization during the cell-division cycle is summarized and discussed. Although the Ki-67 protein is well characterized on the molecular level and extensively used as a proliferation marker, the functional significance still remains unclear. There are indications, however, that Ki-67 protein expression is an absolute requirement for progression through the cell-division cycle.
Copyright 2000 Wiley-Liss, Inc.
Similar articles
- [In situ determination of the Ki-67 growth fraction (Ki-67 GF) in human tumors (studies in breast cancer)].
Lellé RJ. Lellé RJ. Acta Histochem Suppl. 1990;39:109-24. Acta Histochem Suppl. 1990. PMID: 2080254 Review. German. - Prognostic significance of p53, bcl-2 and Ki-67 in high risk superficial bladder cancer.
Stavropoulos NE, Filiadis I, Ioachim E, Hastazeris K, Tsimaris I, Kalogeras D, Stefanaki S, Agnantis NJ. Stavropoulos NE, et al. Anticancer Res. 2002 Nov-Dec;22(6B):3759-64. Anticancer Res. 2002. PMID: 12552989 - Prognostic significance of expressions of cell-cycle regulatory proteins in gastrointestinal stromal tumor and the relevance of the risk grade.
Nakamura N, Yamamoto H, Yao T, Oda Y, Nishiyama K, Imamura M, Yamada T, Nawata H, Tsuneyoshi M. Nakamura N, et al. Hum Pathol. 2005 Jul;36(7):828-37. doi: 10.1016/j.humpath.2005.03.012. Hum Pathol. 2005. PMID: 16084954 - Prognostic significance of p27KIP1 protein and ki-67 growth fraction in non-small cell lung cancers.
Hommura F, Dosaka-Akita H, Mishina T, Nishi M, Kojima T, Hiroumi H, Ogura S, Shimizu M, Katoh H, Kawakami Y. Hommura F, et al. Clin Cancer Res. 2000 Oct;6(10):4073-81. Clin Cancer Res. 2000. PMID: 11051259
Cited by
- Surrogate Immunohistochemical Markers of Proliferation and Embryonic Stem Cells in Distinguishing Ameloblastoma from Ameloblastic Carcinoma.
Robinson L, Smit C, van Heerden MB, Moolla H, Afrogheh AH, Opperman JF, Ambele MA, van Heerden WFP. Robinson L, et al. Head Neck Pathol. 2024 Oct 4;18(1):92. doi: 10.1007/s12105-024-01704-8. Head Neck Pathol. 2024. PMID: 39365497 Free PMC article. - Multiomics and single-cell sequencings reveal the specific biological characteristics of low Ki-67 triple-negative breast cancer.
Han B, Han X, Luo H, Nasir J, Chen C, Shao Z, Ling H, Hu X. Han B, et al. Cancer Innov. 2024 Sep 19;3(5):e146. doi: 10.1002/cai2.146. eCollection 2024 Oct. Cancer Innov. 2024. PMID: 39301202 Free PMC article. - A novel amphiphilic squalene-based compound with open-chain polyethers reduces malignant melanoma metastasis in-vitro and in-vivo.
Zhang Y, Bejaoui M, Linh TN, Arimura T, Isoda H. Zhang Y, et al. Cell Commun Signal. 2024 Sep 11;22(1):437. doi: 10.1186/s12964-024-01813-5. Cell Commun Signal. 2024. PMID: 39261954 Free PMC article. - Patterned PVA Hydrogels with 3D Petri Dish® Micro-Molds of Varying Topography for Spheroid Formation of HeLa Cancer Cells: In Vitro Assessment.
Moreno Valtierra M, Urue Corral A, Jiménez-Avalos JA, Barbosa Avalos E, Dávila-Rodríguez J, Morales Hernández N, Comas-García M, Toriz González G, Oceguera-Villanueva A, Cruz-Ramos JA, Hernández Gutiérrez R, Martínez Velázquez M, García Carvajal ZY. Moreno Valtierra M, et al. Gels. 2024 Aug 6;10(8):518. doi: 10.3390/gels10080518. Gels. 2024. PMID: 39195047 Free PMC article. - Flow cytometric analysis for Ki67 assessment in formalin-fixed paraffin-embedded breast cancer tissue.
Sato N, Tsujimoto M, Nakatsuji M, Tsuji H, Sugama Y, Shimazu K, Shimoda M, Ishihara H. Sato N, et al. BMC Biol. 2024 Aug 26;22(1):181. doi: 10.1186/s12915-024-01980-4. BMC Biol. 2024. PMID: 39183273 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical