Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion - PubMed (original) (raw)
Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion
D Raucher et al. Cell. 2000.
Free article
Abstract
Binding interactions between the plasma membrane and the cytoskeleton define cell functions such as cell shape, formation of cell processes, cell movement, and endocytosis. Here we use optical tweezers tether force measurements and show that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP2) acts as a second messenger that regulates the adhesion energy between the cytoskeleton and the plasma membrane. Receptor stimuli that hydrolyze PIP2 lowered adhesion energy, a process that could be mimicked by expressing PH domains that sequester PIP2 or by targeting a 5'-PIP2-phosphatase to the plasma membrane to selectively lower plasma membrane PIP2 concentration. Our study suggests that plasma membrane PIP2 controls dynamic membrane functions and cell shape by locally increasing and decreasing the adhesion between the actin-based cortical cytoskeleton and the plasma membrane.
Similar articles
- PIP3, PIP2, and cell movement--similar messages, different meanings?
Insall RH, Weiner OD. Insall RH, et al. Dev Cell. 2001 Dec;1(6):743-7. doi: 10.1016/s1534-5807(01)00086-7. Dev Cell. 2001. PMID: 11740936 Free PMC article. Review. - Membrane cytoskeleton: PIP(2) pulls the strings.
Nebl T, Oh SW, Luna EJ. Nebl T, et al. Curr Biol. 2000 May 4;10(9):R351-4. doi: 10.1016/s0960-9822(00)00465-6. Curr Biol. 2000. PMID: 10801429 Review. - Phosphatidylinositol 4,5-bisphosphate phosphatase regulates the rearrangement of actin filaments.
Sakisaka T, Itoh T, Miura K, Takenawa T. Sakisaka T, et al. Mol Cell Biol. 1997 Jul;17(7):3841-9. doi: 10.1128/MCB.17.7.3841. Mol Cell Biol. 1997. PMID: 9199318 Free PMC article. - Chapter 17: Application of laser tweezers to studies of membrane-cytoskeleton adhesion.
Raucher D. Raucher D. Methods Cell Biol. 2008;89:451-66. doi: 10.1016/S0091-679X(08)00617-1. Methods Cell Biol. 2008. PMID: 19118686
Cited by
- Phosphatidylinositol 4,5-Bisphosphate Modulates the Affinity of Talin-1 for Phospholipid Bilayers and Activates Its Autoinhibited Form.
Ye X, McLean MA, Sligar SG. Ye X, et al. Biochemistry. 2016 Sep 13;55(36):5038-48. doi: 10.1021/acs.biochem.6b00497. Epub 2016 Aug 31. Biochemistry. 2016. PMID: 27548281 Free PMC article. - Feedback regulation between plasma membrane tension and membrane-bending proteins organizes cell polarity during leading edge formation.
Tsujita K, Takenawa T, Itoh T. Tsujita K, et al. Nat Cell Biol. 2015 Jun;17(6):749-58. doi: 10.1038/ncb3162. Epub 2015 May 4. Nat Cell Biol. 2015. PMID: 25938814 - Phosphatidylinositol-4,5 bisphosphate produced by PIP5KIgamma regulates gelsolin, actin assembly, and adhesion strength of N-cadherin junctions.
El Sayegh TY, Arora PD, Ling K, Laschinger C, Janmey PA, Anderson RA, McCulloch CA. El Sayegh TY, et al. Mol Biol Cell. 2007 Aug;18(8):3026-38. doi: 10.1091/mbc.e06-12-1159. Epub 2007 May 30. Mol Biol Cell. 2007. PMID: 17538019 Free PMC article. - Ca2+/calmodulin disrupts AKAP79/150 interactions with KCNQ (M-Type) K+ channels.
Bal M, Zhang J, Hernandez CC, Zaika O, Shapiro MS. Bal M, et al. J Neurosci. 2010 Feb 10;30(6):2311-23. doi: 10.1523/JNEUROSCI.5175-09.2010. J Neurosci. 2010. PMID: 20147557 Free PMC article. - Control of cortical rigidity by the cytoskeleton: emerging roles for septins.
Gilden J, Krummel MF. Gilden J, et al. Cytoskeleton (Hoboken). 2010 Aug;67(8):477-86. doi: 10.1002/cm.20461. Cytoskeleton (Hoboken). 2010. PMID: 20540086 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials