The right end of the vir region of an octopine-type Ti plasmid contains four new members of the vir regulon that are not essential for pathogenesis - PubMed (original) (raw)

The right end of the vir region of an octopine-type Ti plasmid contains four new members of the vir regulon that are not essential for pathogenesis

V S Kalogeraki et al. J Bacteriol. 2000 Mar.

Abstract

We sequenced the virD-virE, virE-virF, and virF-T-DNA intergenic regions of an octopine Ti plasmid. Four newly described genes were induced by the vir gene inducer acetosyringone, two of which are conserved in the nopaline-type Ti plasmid pTiC58. One gene resembles a family of phosphatase genes. Each of these genes is dispensable for tumorigenesis.

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Figures

FIG. 1

FIG. 1

Genetic map of the right end of the vir region. virD1 to -4, virE1 and -2, and virF have been previously described. Newly described genes that are induced more than fivefold by vir gene-inducing stimuli are designated vir genes; these include virD5, virE3, virP, and virR. All other open reading frames are designated ORFs. Shaded bars designate newly sequenced regions.

FIG. 2

FIG. 2

Nuclease S1 protection assays of ORFs described in this study. Lanes 1, synthetic radiolabelled oligonucleotide in the absence of mRNA or nuclease S1. Lanes 2 to 4, nuclease S1-resistant oligonucleotides after hybridization with mRNA from a virG+ strain cultured without acetosyringone (lane 2), from a virG+ strain cultured with 100 μM acetosringone (lane 3), or from a virG mutant strain cultured with 100 μM acetosyringone (lane 4).

FIG. 3

FIG. 3

A repeated amino acid sequence motif found within the VirD5 protein. The dot matrix plot of VirD5 against itself (A) was obtained using GenePro (Riverside Scientific Enterprises), while the alignment of these repeated sequences (B) was obtained using MegAlign (DNASTAR, Inc).

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