Evaluation of the discriminatory powers of the Dienes test and ribotyping as typing methods for Proteus mirabilis - PubMed (original) (raw)

Comparative Study

Evaluation of the discriminatory powers of the Dienes test and ribotyping as typing methods for Proteus mirabilis

M A Pfaller et al. J Clin Microbiol. 2000 Mar.

Abstract

A total of 63 clinical isolates of Proteus mirabilis collected over a 19-month period were typed by the Dienes test and ribotyping. Ribotyping was performed using the fully automated RiboPrinter Microbial Characterization System (Qualicon, Wilmington, Del.). Isolates that were indistinguishable by the Dienes test and/or ribotyping were characterized further by pulsed-field gel electrophoresis (PFGE). Most of the isolates represented unique strains as judged by the Dienes test and ribotyping. Forty isolates represented 40 different ribotypes and Dienes types. The remaining 23 isolates were grouped into 13 Dienes types, 12 ribotypes, and 14 PFGE types. The index of discrimination was 0.980 for the Dienes test, 0.979 for ribotyping, and 0.992 for PFGE. Both the Dienes test and ribotyping are useful methods for identifying individual strains of P. mirabilis. The Dienes test is simple, inexpensive, and easy to perform. It can be performed in virtually any laboratory and should be used in the initial epidemiologic characterization of P. mirabilis isolates.

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Figures

FIG. 1

FIG. 1

Dienes test showing three different strains, A, B, and C. Note the absence of Dienes lines between identical strains A and A, B and B, and C and C, respectively. Likewise, note the distinct lines of demarcation between strains A, B, and C.

FIG. 2

FIG. 2

Ribotype profiles of 10 different isolates of P. mirabilis. Lane 1, ribotype 851.4; lane 2, ribotype 851.8; lanes 3, 8, and 10, ribotype 855.7; lanes 4 and 6, ribotype 858.2; lane 5, ribotype 859.5; lanes 7 and 9, ribotype 637.8. Molecular size markers in terms of kilobase pairs are shown at the left of the figure.

FIG. 3

FIG. 3

PFGE profiles of 10 different isolates of P. mirabilis (same order as in Fig. 2). Lane 1, PFGE type 16; lane 2, PFGE type 3; lanes 3, 8, and 10, PFGE type 4; lane 4, PFGE type 8; lane 5, PFGE type 17; lane 6, PFGE type 6; lane 7, PFGE type 11; lane 9, PFGE type 9.

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