Regulation of cytokinesis by the Elm1 protein kinase in Saccharomyces cerevisiae - PubMed (original) (raw)
. 2000 Apr:113 ( Pt 8):1435-45.
doi: 10.1242/jcs.113.8.1435.
Affiliations
- PMID: 10725226
- DOI: 10.1242/jcs.113.8.1435
Regulation of cytokinesis by the Elm1 protein kinase in Saccharomyces cerevisiae
N Bouquin et al. J Cell Sci. 2000 Apr.
Abstract
A Saccharomyces cerevisiae mutant unable to grow in a cdc28-1N background was isolated and shown to be affected in the ELM1 gene. Elm1 is a protein kinase, thought to be a negative regulator of pseudo-hyphal growth. We show that Cdc11, one of the septins, is delocalised in the mutant, indicating that septin localisation is partly controlled by Elm1. Moreover, we show that cytokinesis is delayed in an elm1delta mutant. Elm1 levels peak at the end of the cell cycle and Elm1 is localised at the bud neck in a septin-dependent fashion from bud emergence until the completion of anaphase, at about the time of cell division. Genetic and biochemical evidence suggest that Elm1 and the three other septin-localised protein kinases, Hsl1, Gin4 and Kcc4, work in parallel pathways to regulate septin behaviour and cytokinesis. In addition, the elm1delta;) morphological defects can be suppressed by deletion of the SWE1 gene, but not the cytokinesis defect nor the septin mislocalisation. Our results indicate that cytokinesis in budding yeast is regulated by Elm1.
Similar articles
- Hsl7 localizes to a septin ring and serves as an adapter in a regulatory pathway that relieves tyrosine phosphorylation of Cdc28 protein kinase in Saccharomyces cerevisiae.
Shulewitz MJ, Inouye CJ, Thorner J. Shulewitz MJ, et al. Mol Cell Biol. 1999 Oct;19(10):7123-37. doi: 10.1128/MCB.19.10.7123. Mol Cell Biol. 1999. PMID: 10490648 Free PMC article. - Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast.
Barral Y, Parra M, Bidlingmaier S, Snyder M. Barral Y, et al. Genes Dev. 1999 Jan 15;13(2):176-87. doi: 10.1101/gad.13.2.176. Genes Dev. 1999. PMID: 9925642 Free PMC article. - Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck.
Merlini L, Fraschini R, Boettcher B, Barral Y, Lucchini G, Piatti S. Merlini L, et al. PLoS Genet. 2012;8(4):e1002670. doi: 10.1371/journal.pgen.1002670. Epub 2012 Apr 26. PLoS Genet. 2012. PMID: 22570619 Free PMC article. - Regulation of septin organization and function in yeast.
Longtine MS, Bi E. Longtine MS, et al. Trends Cell Biol. 2003 Aug;13(8):403-9. doi: 10.1016/s0962-8924(03)00151-x. Trends Cell Biol. 2003. PMID: 12888292 Review. - The septin cortex at the yeast mother-bud neck.
Gladfelter AS, Pringle JR, Lew DJ. Gladfelter AS, et al. Curr Opin Microbiol. 2001 Dec;4(6):681-9. doi: 10.1016/s1369-5274(01)00269-7. Curr Opin Microbiol. 2001. PMID: 11731320 Review.
Cited by
- Septin Organization and Dynamics for Budding Yeast Cytokinesis.
Varela Salgado M, Piatti S. Varela Salgado M, et al. J Fungi (Basel). 2024 Sep 9;10(9):642. doi: 10.3390/jof10090642. J Fungi (Basel). 2024. PMID: 39330402 Free PMC article. Review. - Reciprocal regulation by Elm1 and Gin4 controls septin hourglass assembly and remodeling.
Marquardt J, Chen X, Bi E. Marquardt J, et al. J Cell Biol. 2024 May 6;223(5):e202308143. doi: 10.1083/jcb.202308143. Epub 2024 Mar 5. J Cell Biol. 2024. PMID: 38448162 Free PMC article. - Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesis.
Okada H, Chen X, Wang K, Marquardt J, Bi E. Okada H, et al. bioRxiv [Preprint]. 2023 Nov 8:2023.11.07.566094. doi: 10.1101/2023.11.07.566094. bioRxiv. 2023. PMID: 37986946 Free PMC article. Preprint. - Elucidating the Synergistic Role of Elm1 and Gin4 Kinases in Regulating Septin Hourglass Assembly.
Marquardt J, Chen X, Bi E. Marquardt J, et al. bioRxiv [Preprint]. 2023 Nov 8:2023.11.08.566235. doi: 10.1101/2023.11.08.566235. bioRxiv. 2023. PMID: 37986786 Free PMC article. Preprint. - Simultaneous co-overexpression of Saccharomyces cerevisiae septins Cdc3 and Cdc10 drives pervasive, phospholipid-, and tag-dependent plasma membrane localization.
Benson A, McMurray M. Benson A, et al. Cytoskeleton (Hoboken). 2023 Jul-Aug;80(7-8):199-214. doi: 10.1002/cm.21762. Epub 2023 Apr 26. Cytoskeleton (Hoboken). 2023. PMID: 37098755 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases