Age-related alterations in the activation of heat shock transcription factor 1 in rat hepatocytes - PubMed (original) (raw)

. 2000 Apr 10;256(1):83-93.

doi: 10.1006/excr.2000.4808.

Affiliations

• PMID: 10739655
• DOI: 10.1006/excr.2000.4808

Age-related alterations in the activation of heat shock transcription factor 1 in rat hepatocytes

A R Heydari et al. Exp Cell Res. 2000.

Abstract

The induction of hsp70 transcription by heat shock is significantly reduced in hepatocytes isolated from old rats compared to hepatocytes isolated from young/adult rats, and the decline in hsp70 transcription is correlated with a decrease in the induction of heat shock transcription factor 1 (HSF1) binding to the heat shock element. However, the decreased HSF1 binding activity to DNA is not due to reduced levels of HSF1 that are available for activation by heat shock. In fact, the levels of HSF1 are two- to threefold higher in hepatocytes from old rats, and the age-related increase in the levels of HSF1 protein in hepatocytes appears to arise from a decrease in the degradation of the HSF1 because HSF1 mRNA levels do not change and the synthesis of HSF1 decreases approximately 50% with age. No evidence was found for an impairment in HSF1 oligomerization in hepatocytes from old rats, e.g., the level of HSF1 trimers, the nuclear translocation of HSF1, and the phosphorylation of HSF1 after heat shock are similar in hepatocytes isolated from young/adult and old rats. However, the thermostability of the DNA binding activity of HSF1 was significantly reduced with age in a cell-free system as well as in isolated hepatocytes.

Copyright 2000 Academic Press.

PubMed Disclaimer

Similar articles

• Effect of caloric restriction on the expression of heat shock protein 70 and the activation of heat shock transcription factor 1.
  Heydari AR, You S, Takahashi R, Gutsmann A, Sarge KD, Richardson A. Heydari AR, et al. Dev Genet. 1996;18(2):114-24. doi: 10.1002/(SICI)1520-6408(1996)18:2<114::AID-DVG4>3.0.CO;2-C. Dev Genet. 1996. PMID: 8934873
• The expression of heat shock protein 70 decreases with age in lymphocytes from rats and rhesus monkeys.
  Pahlavani MA, Harris MD, Moore SA, Weindruch R, Richardson A. Pahlavani MA, et al. Exp Cell Res. 1995 May;218(1):310-8. doi: 10.1006/excr.1995.1160. Exp Cell Res. 1995. PMID: 7737368
• Hsp70 and aging.
  Heydari AR, Takahashi R, Gutsmann A, You S, Richardson A. Heydari AR, et al. Experientia. 1994 Nov 30;50(11-12):1092-8. doi: 10.1007/BF01923466. Experientia. 1994. PMID: 7988669 Review.
• Sensing stress and responding to stress.
  Voellmy R. Voellmy R. EXS. 1996;77:121-37. doi: 10.1007/978-3-0348-9088-5_9. EXS. 1996. PMID: 8856972 Review.

Cited by

• The age-related failure of adaptive responses to contractile activity in skeletal muscle is mimicked in young mice by deletion of Cu,Zn superoxide dismutase.
  Vasilaki A, van der Meulen JH, Larkin L, Harrison DC, Pearson T, Van Remmen H, Richardson A, Brooks SV, Jackson MJ, McArdle A. Vasilaki A, et al. Aging Cell. 2010 Dec;9(6):979-90. doi: 10.1111/j.1474-9726.2010.00635.x. Epub 2010 Oct 29. Aging Cell. 2010. PMID: 20883524 Free PMC article.
• Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1.
  Wang X, Grammatikakis N, Siganou A, Calderwood SK. Wang X, et al. Mol Cell Biol. 2003 Sep;23(17):6013-26. doi: 10.1128/MCB.23.17.6013-6026.2003. Mol Cell Biol. 2003. PMID: 12917326 Free PMC article.
• Contesting the dogma of an age-related heat shock response impairment: implications for cardiac-specific age-related disorders.
  Carnemolla A, Labbadia JP, Lazell H, Neueder A, Moussaoui S, Bates GP. Carnemolla A, et al. Hum Mol Genet. 2014 Jul 15;23(14):3641-56. doi: 10.1093/hmg/ddu073. Epub 2014 Feb 19. Hum Mol Genet. 2014. PMID: 24556212 Free PMC article.
• Increased age reduces DAF-16 and SKN-1 signaling and the hormetic response of Caenorhabditis elegans to the xenobiotic juglone.
  Przybysz AJ, Choe KP, Roberts LJ, Strange K. Przybysz AJ, et al. Mech Ageing Dev. 2009 Jun;130(6):357-69. doi: 10.1016/j.mad.2009.02.004. Epub 2009 Mar 13. Mech Ageing Dev. 2009. PMID: 19428455 Free PMC article.
• Restoring HSP70 deficiencies improves glucose tolerance in diabetic monkeys.
  Kavanagh K, Flynn DM, Jenkins KA, Zhang L, Wagner JD. Kavanagh K, et al. Am J Physiol Endocrinol Metab. 2011 May;300(5):E894-901. doi: 10.1152/ajpendo.00699.2010. Epub 2011 Feb 15. Am J Physiol Endocrinol Metab. 2011. PMID: 21325107 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Medical

  • MedlinePlus Health Information